Francesca M Storici

US Patent:

20040171154, Sep 2, 2004

Filed:

Jan 26, 2004

Appl. No.:

10/484989

Inventors:

Francesca Storici - Durham NC, US
Lysle Lewis - San Marcos TX, US
Michael Resnick - Chapel Hill NC, US

International Classification:

C12N015/85
C12Q001/68

US Classification:

435/455000, 435/006000

Abstract:

This disclosure provides several methods to generate nucleic acid mutations in vivo, for instance in such a way that no heterologous sequence is retained after the mutagenesis is complete. The methods employ integrative recombinant oligonucleotides (IROs). Specific examples of the described mutagenesis methods enable site-specific point mutations, deletions, and insertions. Also provided are methods that enable multiple rounds of mutation and random mutagenesis in a localized region. The described methods are applicable to any organism that has a homologous recombination system.