Frederick W Reynolds

US Patent:

52178960, Jun 8, 1993

Filed:

Dec 30, 1988

Appl. No.:

7/292263

Inventors:

Steven P. Kramer - Kew Gardens NY
David M. Valenzuela - Franklin Square NY
Frederick H. Reynolds - Syosset NY
John M. Sorvillo - Merrick NY

Assignee:

Oncogene Science, Inc. - Uniondale NY

International Classification:

C12N 520
C12N 1502
C07K 1528
C12P 2108

US Classification:

43524027

Abstract:

This invention provides a monoclonal antibody which specifically forms a complex with amino acids 1-87 of PTHLP which does not form a complex with amino acids 1-34 of PTHLP, and which forms a complex with the epitope to which any of the monoclonal antibodies produced by the hybridomas 212-10. 7, (ATCC Accession No. HB9930), 199-999 (ATCC Accession No. HB9929), 199-278 (ATCC Accession No. HB9931), is directed. This invention further provides methods of detecting PTHLP and of diagnosing and treating humoral hypercalcemia of malignancy.

US Patent:

51908581, Mar 2, 1993

Filed:

Feb 1, 1989

Appl. No.:

7/304947

Inventors:

John M. Sorvillo - Merrick NY
David M. Valenzuela - Franklin Square NY
Frederick H. Reynolds - Syosset NY

Assignee:

Oncogene Science, Inc. - Uniondale NY

International Classification:

C12Q 100
G01N 3353

US Classification:

435 723

Abstract:

The present invention provides a monoclonal antibody which specifically forms a complex with TGF. alpha. in formalin-fixed, paraffin-embedded tissue sections which has an affinity of a least 10. sup. 7, and which is directed to the epitope to which monoclonal antibody 213-4. 4 (ATCC No. HB9992) is directed. The invention further provides the above-described monoclonal antibody wherein the epitope consists essentially of amino acids 34-43 of TGF. alpha. Additionally, the invention concerns the monoclonal antibody 213-4. 4 (ATCC No. HB9992). The invention also provides a method of detecting TGF. alpha. in tissue sections of a tissue in which normal tissue is characterized by the absence of TGF. alpha. and neoplastic tissue is characterized by the presence of TGF. alpha. in a subset of such neoplastic tissue which comprises contacting the tissue sections with an antibody directed to an epitope on TGF. alpha.