Gary Eugene Hughes

US Patent:

20200408710, Dec 31, 2020

Filed:

Jun 26, 2020

Appl. No.:

16/913704

Inventors:

- Whitestown IN, US
Christopher Dailey - Whitestown IN, US
Gary L. Hughes - Camby IN, US

International Classification:

G01N 27/327

Abstract:

In one embodiment, a system for detecting an analyte in a bodily fluid sample includes an electrochemical biosensor, the electrochemical biosensor configured to receive the bodily fluid sample, test for the analyte, and produce an analyte level for the analyte. The system further includes a mediator, the mediator configured to provide for the testing of the analyte by facilitating electron exchange between the analyte and the biosensor. The system further includes an oxygen tension reducing agent, the oxygen tension reducing agent reducing interference of oxygen with the operation of the mediator.

US Patent:

20200300836, Sep 24, 2020

Filed:

Oct 14, 2019

Appl. No.:

16/601433

Inventors:

- Whitestown IN, US
Gary L. Hughes - Camby IN, US
Keith Moskowitz - Westfield IN, US

Assignee:

POLYMER TECHNOLOGY SYSTEMS, INC. - Whitestown IN

International Classification:

G01N 33/487
G01N 21/64
G01N 33/49
G01N 27/327
G01N 27/30

Abstract:

Systems and methods for point-of-care determination of blood potassium are provided. The approaches are based on the reaction of ADP and PEP in the presence of potassium ion and pyruvate kinase to produce pyruvate and ATP. In one method, the produced pyruvate is reacted with phosphate and mediator, in the presence of pyruvate oxidase, to yield acetylphosphate and reduced mediator, and the reduced mediator is used to determine the blood potassium using conventional electrochemical methods. In an alternative method, the produced pyruvate is reacted with phosphate and oxygen in the presence of pyruvate oxidase to yield acetylphosphate and hydrogen peroxide, and the hydrogen peroxide is measured optically in accordance with known methods.

US Patent:

20200174024, Jun 4, 2020

Filed:

Nov 27, 2019

Appl. No.:

16/698872

Inventors:

- Whitestown IN, US
Brittney L. Werner - Fairland IN, US
Gary L. Hughes - Camby IN, US
Jie Chen - Whitestown IN, US

International Classification:

G01N 33/72
G01N 27/327
C12N 9/50

Abstract:

A method is disclosed comprising lysing the red blood cells of a whole blood sample, oxidizing the free hemoglobin in the lysed sample, and cleaving FVH from the hemoglobin A1C to form an electrochemical test solution. In one aspect, a first portion of the electrochemical test solution is reacted with fructosyl peptide oxidase and a reduced ruthenium mediator to form a first reaction product. A first electrical property of the first reaction product is measured, the measurement being indicative of hemoglobin A1C in the blood sample. In another aspect, a second portion of the electrochemical test solution is reacted with ferrocyanide to form a second reaction product. A second electrical property of the second reaction product is measured, the measurement being indicative of total hemoglobin in the blood sample. Hemoglobin A1C, total hemoglobin, and % HbA1C are determined based on the first and second electrical properties. Also provided are systems and components useful in performing the disclosed methods.

US Patent:

20190212290, Jul 11, 2019

Filed:

Jan 10, 2019

Appl. No.:

16/245063

Inventors:

- Indianapolis IN, US
Gary Hughes - Camby IN, US

International Classification:

G01N 27/327
C12N 9/04
G01N 33/543

Abstract:

A system for the electrochemical detection of analyte levels includes a test strip including an electrode and a counter electrode, the electrode and counter electrode located proximate to a sample reception area. The system further includes a coating on one of the electrode and counter electrode, the coating including a reagent coating for an analyte. The reagent coating includes a creatinine iminohydrolase, deamido NAD, ATP, and NAD Synthetase/Mg.

US Patent:

20190011389, Jan 10, 2019

Filed:

Aug 7, 2017

Appl. No.:

15/670499

Inventors:

- INDIANAPOLIS IN, US
Aniruddha Patwardhan - Fishers IN, US
Gary Hughes - Camby IN, US

International Classification:

G01N 27/327
C12Q 1/00

Abstract:

An electrochemical test for total cholesterol includes a test strip for an electrochemical testing testing of a blood analyte which includes a first receiving port, the first receiving port for receiving a blood sample, the first receiving port at a first end of the test strip. The test strip further includes a first electrode and a second electrode, the first and second electrodes proximate to the first receiving port. The test strip further includes a first contact and a second contact, the first and second contacts at a second end of the test strip, the first and second contacts interconnected with the first and second electrodes, respectively. The test strip further includes cholesterol oxidase, located proximate to the first and the second electrode. The test strip further includes a mediator, located proximate to the first and the second electrode, wherein the cholesterol oxidase and the mediator interact with the blood sample and the first and second electrode to generate a measurable electrical event.

US Patent:

20170299539, Oct 19, 2017

Filed:

Apr 12, 2017

Appl. No.:

15/485871

Inventors:

- Indianapolis IN, US
Gary L. Hughes - Camby IN, US

International Classification:

G01N 27/327
G01N 33/92
C12Q 1/00
G01N 33/543

Abstract:

A system for the electrochemical detection of triglyceride levels includes a test strip including an electrode and a counter electrode, the electrode and counter electrode located proximate to a sample reception area; and a coating on one of the electrode and counter electrode, the coating including a reagent coating for triglycerides.

US Patent:

20170176472, Jun 22, 2017

Filed:

Dec 16, 2016

Appl. No.:

15/382367

Inventors:

- Indianapolis IN, US
Gary L. Hughes - Camby IN, US

International Classification:

G01N 33/92

Abstract:

A system for testing for LDL-Particles (LDL-P) and HDL-Particles (HDL-P) includes a first lateral flow test strip and a second lateral flow test strip; and a dosing area, the dosing area interconnected with the first and second lateral flow test strips. The system further includes a collector for collecting a sample; and a first mixer for receiving the sample from the collector, the mixer including buffers for mixing with the sample, the first mixer for dosing the sample pad, wherein the first lateral flow test strip provides for the detection of HDL-P and the second lateral flow test strip provides for the detection of LDL-P.

US Patent:

20170089894, Mar 30, 2017

Filed:

Sep 27, 2016

Appl. No.:

15/277510

Inventors:

- Indianapolis IN, US
Gary L. Hughes - Camby IN, US

International Classification:

G01N 33/558
G01N 33/92

Abstract:

A system for testing for LDL-C and LDL-P includes a first lateral flow test strip, a second lateral flow test strip, and a dosing area, the dosing area interconnected with the first and second lateral flow test strips. The system further includes a collector for collecting a sample and a first mixer for receiving the sample from the collector, the mixer including buffers for mixing with the sample, the first mixer for dosing the sample pad a first time. The first lateral flow test strip provides for the detection of LDL-C, and the second lateral flow test strip provides for the detection of LDL-P.