Giovanni Zocchi

US Patent:

8460870, Jun 11, 2013

Filed:

Jul 20, 2011

Appl. No.:

13/187351

Inventors:

Giovanni Zocchi - Los Angeles CA, US
Brian Choi - Los Angeles CA, US

Assignee:

The Regents of the University of California - Oakland CA

International Classification:

C12Q 1/68
C12Q 1/48
C12P 21/00
C12N 9/00
C12N 9/12
C07K 2/00
C07K 1/00
C07H 21/00

US Classification:

435 61, 435183, 435194, 435 691, 435 15, 530402, 530350, 536 231

Abstract:

A method of altering the conformation of a polypeptide having a known three-dimensional structure is described. The method comprises attaching a first end of a polymer to a first portion of the polypeptide, attaching a second end of the polymer to a second portion of the polypeptide, and altering the mechanical tension of the polymer, thereby altering the conformation of the polypeptide. The alteration of the conformation of the polypeptide may increase or decrease the binding affinity of the polypeptide for a substrate bound by the polypeptide, or alter the catalytic rate of an enzyme. Typically, the polymer is a polynucleotide or polypeptide.

US Patent:

20040241699, Dec 2, 2004

Filed:

Apr 1, 2004

Appl. No.:

10/491353

Inventors:

Giovanni Zocchi - Los Angeles CA, US
Mukta Zocchi - Los Angeles CA, US

International Classification:

C12Q001/68

US Classification:

435/006000

Abstract:

The invention disclosed herein provides a new detection scheme to monitor hybridization between complimentary polynucleotides such as DNA and/or RNA. Embodiments of the invention disclosed herein localized electromagnetic radiation to provide an optimized analysis of polynucleotide hybridization in contexts such as the polynucleotide microarrays typically used on gene chips.

US Patent:

20080311592, Dec 18, 2008

Filed:

Jan 20, 2006

Appl. No.:

11/814275

Inventors:

Giovanni Zocchi - Los Angeles CA, US
Brian Choi - Los Angeles CA, US

Assignee:

The Regents of the University of California - Oakland CA

International Classification:

G01N 33/535
C07K 2/00
C12N 9/96

US Classification:

435 76, 530402, 435188

Abstract:

A method of altering the conformation of a polypeptide having a known three-dimensional structure is described. The method comprises attaching a first end of a polymer to a first portion of the polypeptide, attaching a second end of the polymer to a second portion of the polypeptide, and altering the mechanical tension of the polymer, thereby altering the conformation of the polypeptide. The alteration of the conformation of the polypeptide may increase or decrease the binding affinity of the polypeptide for a substrate bound by the polypeptide, or alter the catalytic rate of an enzyme. Typically, the polymer is a polynucleotide or polypeptide.