Gregory O Payne

US Patent:

7288532, Oct 30, 2007

Filed:

Aug 20, 1999

Appl. No.:

09/763324

Inventors:

Gregory F. Payne - Hunt Valley MD, US
Guneet Kumar - Ellicott City MD, US

Assignee:

The University of Maryland Biotechnology Institute - Rockville MD
The University of Maryland, Baltimore County - Baltimore MD

International Classification:

C08G 8/00
C12P 19/04

US Classification:

514 55, 435 681, 435 72, 435 74, 435101, 527200, 527206, 527303, 536 20, 536 551, 536 553, 536127

Abstract:

The present invention is directed to homogeneous phase enzyme-catalyzed processes for producing modified chitosan polymers or oligomers. An enzyme is reacted with a phenolic substrate in the presence of a chitosan polymer or oligomer to produce a modified chitosan polymer or oligomer. The invention also includes modified chitosan polymers or oligomers produced by the novel processes, in particular modified chitosan polymers or oligomers having useful functional properties, such as base solubility and/or high viscosity.

US Patent:

7790010, Sep 7, 2010

Filed:

Jun 17, 2005

Appl. No.:

11/155116

Inventors:

William E. Bentley - Annapolis MD, US
Reza Ghodssi - Rockville MD, US
Gregory F. Payne - Cockeysville MD, US
Gary W. Rubloff - Clarksville MD, US
Li-Qun Wu - North Potomac MD, US
Hyunmin Yi - Ellicott City MD, US
Wolfgang Losert - Bethesda MD, US
Douglas S. English - Silver Spring MD, US

Assignee:

University of Maryland, College Park - College Park MD
University of Maryland, Baltimore County - Baltimore MD

International Classification:

C25D 5/02
C25D 5/48
C25D 9/02

US Classification:

205118, 205136, 205229, 205317

Abstract:

A method is provided for electrochemically depositing a polymer with spatial selectivity. A substrate having a substrate surface is contacted with an aqueous solution containing a selectively insolubilizable polysaccharide, such as chitosan, which is subjected to electrochemically treatment to deposit, with spatial selectivity, the selectively insolubilizable polysaccharide on a patterned electrically conductive portion of the substrate surface.

US Patent:

7820227, Oct 26, 2010

Filed:

Feb 20, 2004

Appl. No.:

10/581905

Inventors:

Gregory F. Payne - Cockeysville MD, US
Gary W. Rubloff - Clarksville MD, US
Hyunmin Yi - Lexington MA, US
Rohan Fernandes - Beltsville MD, US
Li-Qun Wu - North Potomac MD, US
Reza Ghodssi - Silver Spring MD, US
William E. Bentley - Annapolis MD, US

Assignee:

University of Maryland, College Park - College Park MD
University of Maryland, Baltimore County - Baltimore MD

International Classification:

A61L 33/00

US Classification:

427 21, 427 211, 427 212, 427299, 427300, 427307, 427322, 427331, 4273722, 4273743, 427384, 4273882, 4273884

Abstract:

A method for biolithographical deposition of molecules is provided. According to an embodiment of the method, a reactive layer (e. g. , a polysaccharide mass) having a surface region coated with a biologically compatible resist is provided. A portion of the biologically compatible resist is selectively removed to expose an exposed portion of the reactive layer. Molecules, such as biomolecules and/or cellular species, are then conjugated to the exposed portion of the reactive layer. Also provided are materials and devices related to the method.

US Patent:

7883615, Feb 8, 2011

Filed:

Feb 11, 2004

Appl. No.:

10/541057

Inventors:

Gregory F. Payne - Cockeysville MD, US
Li-Qun Wu - North Potomac MD, US
Reza Ghodssi - Rockville MD, US
William E. Bentley - Annapolis MD, US
Gary W. Rubloff - Clarksville MD, US
Hyunmin Yi - Ellicott City MD, US
Rohan Fernandes - Beltsville MD, US
Tianhong Chen - Newark DE, US
David A. Small - Silver Spring MD, US

Assignee:

University of Maryland, College Park - College Park MD
University of Maryland, Baltimore County - Baltimore MD

International Classification:

C25D 9/02

US Classification:

205 82, 205317, 205229, 205118

Abstract:

A method is provided for electrochemically depositing a polysaccharide mass having a selected physical state. According to an embodiment, an electrically conductive support of a substrate is contacted with an aqueous solution including a selectively insolubilizable polysaccharide, and the selectively insolubilizable polysaccharide is electrochemically deposited on the electrically conductive support while controlling deposition conditions to form the polysaccharide mass having the selected physical state, such as that of a hydrogel. Deposition may be performed in a spatially and/or temporally controlled manner.

US Patent:

8562809, Oct 22, 2013

Filed:

Oct 2, 2009

Appl. No.:

13/122403

Inventors:

Yi Liu - Bethesda MD, US
Gregory F. Payne - Hunt Valley MD, US
W. Lee Meyer - Baltimore MD, US

Assignee:

University of Maryland, College Park - College Park MD

International Classification:

C25D 9/02

US Classification:

205229, 205317

Abstract:

A method of forming a bioelectronic device including a protein on an electrically conductive substrate, by electrodepositing aminopolysaccharide chitosan on the substrate while applying a cathodic voltage to the substrate, to form an aminopolysaccharide chitosan film thereon, applying an anodic voltage to the substrate in the presence of NaCl to activate the aminopolysaccharide chitosan film so that it is reactive with protein. The method also optionally includes reacting the aminopolysaccharide film, after activation thereof, with the protein, so that the protein assembles on and is coupled to the substrate, thereby forming a bioelectronic device. The protein can include single or multiple protein species, and including biosensing proteins. Additional methods include biosensing of electrochemically active compounds either present in a sample or generated during a biological recognition event and devices useful in such methods. The resulting devices are useful as sensors in hand-held devices, textiles, garments and the like.

US Patent:

20080254104, Oct 16, 2008

Filed:

Mar 17, 2008

Appl. No.:

12/077173

Inventors:

Srinivasa R. Raghavan - Silver Spring MD, US
Gregory F. Payne - Cockeysville MD, US
Chao Zhu - McLean VA, US
Matthew B. Dowling - Washington DC, US

International Classification:

A61K 9/70
A61P 17/02

US Classification:

424445

Abstract:

The present invention provides a novel biomaterial which is a hybrid, self-assembling biopolymeric networked film that is functionalized through hydrophobic interactions with vesicles loaded with bioactive agents. The biomaterial compound is a polymeric network of hydrophobically modified chitosan scaffolds that is taken from solution and formed as a solid film. This solid state film is capable of hydrophobic interactions with the functionalized vesicles. The vesicles include one or more lamellar structures forming one or more nano-compartments that are capable of containing similar or alternative active moieties within. Use of the film results in a degradation of the chitosan scaffold thereby releasing the active moieties within the vesicles from the scaffold. Application of the current invention occurs through various delivery mechanisms and routes of administration as will be described herein.

US Patent:

20110195860, Aug 11, 2011

Filed:

Apr 7, 2009

Appl. No.:

12/990981

Inventors:

Gregory F. Payne - Hunt Valley MD, US
William E. Bentley - St. Michaels MD, US

Assignee:

UNIVERSITY OF MARYLAND, COLLEGE PARK - College Park MD

International Classification:

C40B 30/04
C07K 17/00
C07K 17/10
C40B 40/10
G01N 33/53
C07K 1/107
C07K 1/14

US Classification:

506 9, 530350, 530395, 5303917, 506 18, 436501

Abstract:

Biofunctionalized fibers including a fiber platform and a histidine-tagged protein and, optionally, an antibody. Chitosan is a fiber useful as the fiber platform. The fiber platform may be treated with nickel or may be directly linked to the histidine-tagged protein e.g., histidine-tagged streptococcal IgG-binding protein, protein G, protein G3T, GFP or RFP. The resulting biofunctionalized fibers can be assembled into protein fiber assemblies by a variety of biofabrication methods. The fiber assemblies, e.g., in the form of woven fabrics, are useful for (i) antigen capture; (ii) immunoanalysis, and/or (iii) multiplexed analysis. In one fabrication, each fiber of a fiber assembly presents a specific antibody, and mixing and matching of fibers, for example by weaving of fabrics from various antibody-presenting fibers, allows for multiple antigens to be captured simultaneously for multiplexed analysis.

US Patent:

20120103822, May 3, 2012

Filed:

Apr 27, 2010

Appl. No.:

13/266433

Inventors:

Yi Liu - Bethesda MD, US
Gregory F. Payne - Hunt Valley MD, US
Xiaohua Yang - Ardmore OK, US

Assignee:

UNIVERSITY OF MARYLAND COLLEGE PARK - College Park MD

International Classification:

C25D 9/02
G01N 33/53
C12N 13/00

US Classification:

205317, 4351731, 435 71

Abstract:

Methods for the generation of hydrogels formed by electrodeposition of an electroaddressable polymer are described. The hydrogels may contain one or more cell populations electroaddressed or electroaddressable to a location within the hydrogel and where the cells of the cell populations are entrapped by the hydrogel and are capable of expansion within the hydrogel and may be releasable from the hydrogel. Further provided are electroaddressable polysaccharide blends for the in-film expansion of a cell population, allowing probing of the cells and formation of immunocomplexes. Further provided are methods of using hydrogels containing electroaddressed or electroaddressable cell populations in in-film bioprocessing methods such as cell-based biosensing, protein-based biosensing, and in studies of cell signaling.