Juerg F. Tschopp - San Diego CA Gregory P. Thill - San Diego CA Russell A. Brierley - San Diego CA Kathryn A. Barr - Bartlesville OK
Assignee:
Research Corporation Technologies, Inc. - Tucson AZ
International Classification:
C12P 2106 C12N 119 C12N 1500 C12N 1563
US Classification:
435 691
Abstract:
The present invention is directed to expression cassettes comprising a 5' regulatory region from at least one of the Pichia pastoris AOXI gene, p40 gene, DASI gene or HIS4 gene, operably linked to an HSA structural gene including the HSA signal sequence. The HSA structural gene has a translational start codon within 0 to 11 deoxyribonucleotides from the 5' end of the HSA structural gene and is operably linked to a 3' termination sequence. Further, the adenine and thymine content of the intervening deoxyribonucleotides is in the range from about 55 to about 64%. The expression cassette may be an autonomously replicating vector or an integrative vector. Other embodiments of the present invention include Pichia pastoris strains transformed with the HSA expression cassettes and processes for secretion of HSA using the expression cassettes.
Expression Of Hepatitis B S And Pres.sub.2 Proteins In Methylotrophic
Research Corporation Technologies, Inc. - Tucson AZ
International Classification:
C07K 1410 C12N 1581
US Classification:
530403
Abstract:
A process for the enhanced production of antigenic particles consisting essentially of hepatitis B S protein and preS. sub. 2 protein. Also disclosed are novel DNA molecules and hosts transformed with these molecules.
Expression Of Hepatitis B S And Pres.sub.2 Proteins In Pichia Pastoris
Research Corporation Technologies, Inc. - Tucson AZ
International Classification:
C12N 119 C12N 1551 C12N 1581
US Classification:
435 693
Abstract:
A process for the enhanced production of antigenic particles consisting essentially of hepatitis B S protein and preS. sub. 2 protein. Also disclosed are novel DNA molecules and hosts transformed with these molecules.
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