Gretchen P Kiser

US Patent:

20040229222, Nov 18, 2004

Filed:

Apr 1, 2002

Appl. No.:

10/114908

Inventors:

Buena Chui - Chandler AZ, US
Robert Elghanian - Skokie IL, US
Vineet Gupta - Reading MA, US
Krishnamurthy Jayaraman - Hoffman Estates IL, US
Gretchen Kiser - Mesa AZ, US
Changming Li - Schaumburg IL, US
Chang-Gong Liu - Cherry Hill NJ, US
Kenneth Luehrsen - Half Moon Bay CA, US
Abhijit Mazumder - Buffalo Grove IL, US
Ramesh Ramakrishnan - Vernon Hills IL, US
Todd Tuggle - Oceanside CA, US
Carl Yamashiro - Chandler AZ, US
Handy Yowanto - Walnut CA, US
Ekaterina Pestova - Downers Grove IL, US
David Fermin - Minneapolis MN, US
David Wang - Deerfield IL, US
Zhijie Gu - San Diego CA, US

International Classification:

C12Q001/68
G01N033/53
C12M001/34

US Classification:

435/006000, 435/007500, 435/287200

Abstract:

This invention relates to methods and compositions for determining single nucleotide polymorphisms (SNPs) in P450 genes. In preferred embodiments, self extension of interrogation probes is prevented by using novel non self-extension probes and/or methods, thereby improving the specificity and efficiency of P450 SNP detection in target samples with minimal false positive results. The invention thus describes a variety of methods to decrease self-extension of interrogation probes. In addition, this invention provides a unique collection of P450 SNP probes on one assay, primer sequences for specific amplification of each of the seven P450 genes and amplicon control probes to evaluate whether the intended p450 gene targets were amplified successfully. The invention also describes a variety of array platforms for performing the assays of the invention; for example: CodeLink™, eSensor™, multiplex arrays with cartridges etc., all described herein.

Address:

3010 Stanford Ave, Boulder, CO 80305

VIN:

1J8GR48K77C507464

Make:

JEEP

Model:

GRAND CHEROKEE

Year:

2007