Haisun S Liao

US Patent:

20040137439, Jul 15, 2004

Filed:

Jan 10, 2003

Appl. No.:

10/341199

Inventors:

Haisun Liao - Sharon MA, US
Amy Deik - Wakefield MA, US
Natalia Mamaeva - West Roxbury MA, US
Caroline Woodward - Boston MA, US
Yih Huang - Lexington MA, US
Ming Shen - Guilford CT, US
Simon Law - Lexington MA, US

Assignee:

LINDEN TECHNOLOGIES, INC.

International Classification:

C12Q001/68
C12M001/34

US Classification:

435/006000, 435/287200

Abstract:

Disclosed is a method of producing replicates of sample nucleic acids. The method can include providing an insoluble support comprising attached oligonucleotides, annealing sample nucleic acids to the attached oligonucleotides; constructing template nucleic acids by extending the attached oligonucleotides using a polymerase; and transcribing the template nucleic acids to produce RNA replicates of the sample nucleic acids The attached oligonucleotides comprise a promoter sequence and a target annealing sequence, and (2) the proximal end of the promoter sequence is spaced from the insoluble support by a predetermined distance.

US Patent:

20040161792, Aug 19, 2004

Filed:

Mar 31, 2004

Appl. No.:

10/814876

Inventors:

Haisun Liao - Sharon MA, US
Amy Deik - Wakefield MA, US
Natalia Mamaeva - West Roxbury MA, US
Caroline Woodward - Boston MA, US
Yih Huang - Lexington MA, US
Ming Shen - Guilford CT, US
Simon Law - Lexington MA, US

Assignee:

Linden Technologies, Inc., a Delaware corporation

International Classification:

C12Q001/68
C12P019/34

US Classification:

435/006000, 435/091200

Abstract:

Disclosed is a method of producing replicates of sample nucleic acids. The method can include providing an insoluble support comprising attached oligonucleotides, annealing sample nucleic acids to the attached oligonucleotides; constructing template nucleic acids by extending the attached oligonucleotides using a polymerase; and transcribing the template nucleic acids to produce RNA replicates of the sample nucleic acids The attached oligonucleotides comprise a promoter sequence and a target annealing sequence, and (2) the proximal end of the promoter sequence is spaced from the insoluble support by a predetermined distance.

US Patent:

20050064432, Mar 24, 2005

Filed:

Sep 19, 2003

Appl. No.:

10/664608

Inventors:

Simon Law - Lexington MA, US
Haisun Liao - Sharon MA, US

International Classification:

C12Q001/68
C12M001/34

US Classification:

435006000, 435287200

Abstract:

Disclosed is a substrate that includes a promoter primer that can be extended to form a transcribable template nucleic acid; and a capture probe. Typically, the promoter primer and the capture probe are non-complementary, and the capture probe can specifically bind to a target nucleic acid. The substrate can be used to amplify and detect one or more target nucleic acids.