Hanwen Mao

US Patent:

20100099076, Apr 22, 2010

Filed:

Oct 16, 2008

Appl. No.:

12/252623

Inventors:

Hanbin Mao - Kent OH, US
Hanwen Mao - Annandale VA, US
Zian Mao - HangZhou, CN
Jiangsen Mao - HangZhou, CN

Assignee:

Kent State University - Kent OH

International Classification:

C12Q 1/70
C12M 1/00

US Classification:

435 5, 4352871, 4352887

Abstract:

The present system and methods allow for low level detection of as little as single pathogen particles, such as viral or bacterial particles, during the early stage of infection. An optical trapping system, such as laser tweezers, are used to trap a substrate to which an analyte has been bound to detect and record the thermal motion of an antibody-antigen interaction that may occur between an anti-viral antibody-coated microsphere and a viral particle for example. The system may be equipped with a detection system such as a position sensitive photodetector (PSD) to record the thermal motion of a trapped microsphere and particle at a certain frequency. The thermal motion data may be Fourier transformed into a power spectrum, which may be transformed into an output value using a Lorentzian equation. The power spectrum of the trapped microsphere may be recorded before and after binding of the pathogenic particle to determine the presence thereof.

US Patent:

20100183628, Jul 22, 2010

Filed:

Jan 6, 2010

Appl. No.:

12/652877

Inventors:

Michael S. Kinch - Laytonsville MD, US
Michael Goldblatt - McLean VA, US
Douty Bamba - Randallstown MD, US
Shaojing Chang - Rockville MD, US
Huosheng Chen - Germantown MD, US
Zenbework Fesseha - Columbia MD, US
Manu Kohli - Dunn Loring VA, US
Hanwen Mao - Annandale VA, US
Heather Thi Thu Ung-Medoff - Gaithersburg MD, US
Ke Weng - Gaithersburg MD, US

Assignee:

FUNCTIONAL GENETICS, INC. - GAITHERSBURG MD

International Classification:

A61K 39/395
C12Q 1/70
A61P 31/16
A61P 31/18

US Classification:

4241721, 435 5

Abstract:

A method for identifying host genes and encoded proteins for potential targets for therapeutic intervention employs a Gene Search Vector that is either lentivirus or MMLV-based, and can be used to interrogate an entire cell genome without prior knowledge of the genomic sequence. This Random Homozygous Gene Perturbation (RUGP) technique is rapidly verifiable and is used to identify potential host targets for intervention for influenza, HIV and other viral infections. Using Thermal Assymetric Interlaced (TAIL)-PCR, the period for identification of promising targets is reduced from months to weeks or less. Specific targets including PTCH1, Robo1 and Nedd4 are reviewed in detail.

US Patent:

20140271671, Sep 18, 2014

Filed:

Mar 14, 2013

Appl. No.:

13/803877

Inventors:

- Indianapolis IN, US
Michael Goldblatt - McLean VA, US
Wu-Bo Li - North Potomac MD, US
Douty Bamba - Randallstown MD, US
Shaojing Chang - Rockville MD, US
Huosheng Chen - Germantown MD, US
Zenbework Fesseha - Columbia MA, US
Manu Kohli - Dunn Loring VA, US
Hanwen Mao - Annandale VA, US
Heather Thi Thu Ung-Medoff - Gaithersburg MD, US
Ke Weng - Gaithersburg MD, US

Assignee:

Eli Lilly and Company - Indianapolis IN

International Classification:

C12Q 1/68

US Classification:

4241721, 435 5, 435375

Abstract:

A method for identifying host genes and encoded proteins for potential targets for therapeutic intervention employs a Gene Search Vector that is either lentivirus or MMLV-based, and can be used to interrogate an entire cell genome without prior knowledge of the genomic sequence. This Random Homozygous Gene Perturbation (RUGP) technique is rapidly verifiable and is used to identify potential host targets for intervention for influenza, HIV and other viral infections. Using Thermal Assymetric Interlaced (TAIL)-PCR, the period for identification of promising targets is reduced from months to weeks or less. Specific targets including PTCH1, Robo1 and Nedd4 are reviewed in detail.