Helen M Buettner

US Patent:

20030104614, Jun 5, 2003

Filed:

Aug 9, 2002

Appl. No.:

10/215435

Inventors:

Kathryn Uhrich - Plaintield NJ, US
Helen Buettner - West Windsor NJ, US
Kristine Schmalenberg - Dunellen NJ, US

International Classification:

C12N005/02
C12M001/00

US Classification:

435/325000, 435/289100

Abstract:

The present invention is directed to an article which has a pattern of biologically active molecules stably adsorbed directly onto a polymeric substrate. The present invention also provides methods for preparing a pattern of biologically active molecules on the surface of a polymeric substrate, which include exposing a polymeric substrate to conditions that increase the polarity of a surface of the polymeric substrate, and contacting that surface with a stamp that includes a micron-sized pattern coated with biologically active molecules. The present invention also provides a method to spatially modulate the growth of a cell which includes contacting a cell with an article of the present invention for a time and under conditions sufficient to adhere the cell to the biologically active molecules and to grow the cell along the micron-sized pattern of biologically active molecules on the polymeric substrate.