Helena Mathews

US Patent:

6642438, Nov 4, 2003

Filed:

Mar 17, 2000

Appl. No.:

09/527972

Inventors:

Stephanie K. Clendennen - Portland OR
Jill A. Kellogg - Portland OR
Chau B. Phan - Portland OR
Helena V. Mathews - Portland OR
Nancy M. Webb - Portland OR

Assignee:

Exelixis Plant Sciences, Inc. - Portland OR

International Classification:

C12N 1509

US Classification:

800287, 800278, 800298, 800309, 435419, 435468, 4353201, 536 236, 536 241

Abstract:

The present invention is directed to a melon actin promoter capable of providing expression of heterologous genes, and nucleic acid constructs, vectors, transformation methods, transgenic plant cells, and transgenic plants comprising said promoter.

US Patent:

8642340, Feb 4, 2014

Filed:

Dec 17, 2009

Appl. No.:

13/140381

Inventors:

Helena V. Mathews - Portland OR, US
Sung-Yong Yoon - Lake Oswego OR, US
Mylavarapu Venkatramesh - Portland OR, US

Assignee:

Dianaplantsciences, Inc. - Portland OR

International Classification:

C12N 5/00

US Classification:

435420

Abstract:

Disclosed are methods for obtaining a natural product preparation with reduced glucosinolate contamination from a plant of Brassicaceae. The methods can include cultivating a plant callus from a plant capable of producing a desired natural product, selecting a callus with reduced glucosinolate production, and cultivating the selected callus in a liquid medium. The method can also include recovering the natural product from the culture. Also disclosed are methods for obtaining cabbage anthocyanin with reduced glucosinolate contamination. The methods can include cultivating a red cabbage plant callus with reduced glucosinolate production in a liquid medium to obtain a suspension culture and cultivating the suspension culture in a medium lacking a nitrogen source. The method can also include recovering the anthocyanin with reduced glucosinolate contamination from the culture. Finally, several specific low-glucosinolate cell lines are described.

US Patent:

20030182690, Sep 25, 2003

Filed:

May 6, 2003

Appl. No.:

10/431304

Inventors:

Stephanie Clendennen - Portland OR, US
Jill Kellogg - Portland OR, US
Chau Phan - Portland OR, US
Helena Mathews - Portland OR, US
Nancy Webb - Portland OR, US

International Classification:

A01H001/00
C12N015/82
C12N005/04

US Classification:

800/287000, 435/419000, 435/468000

Abstract:

The present invention is directed to banana fruit-associated promoters, a melon actin promoter and banana fusion promoters capable of providing expression of heterologous genes in addition to heterologous nucleic acid constructs, vectors, transformation methods, transgenic plant cells and transgenic plants comprising such promoters. The invention is also directed to methods for screening plant promoters in various types of plant tissue using a transient expression assay.

US Patent:

20120034658, Feb 9, 2012

Filed:

May 4, 2009

Appl. No.:

13/260667

Inventors:

Sung-Yong Yoon - Lake Oswego OR, US
Helena Mathews - Portland OR, US

Assignee:

DIANAPLANTSCIENCES, INC. - Portland OR

International Classification:

C12P 19/60

US Classification:

435 75

Abstract:

Disclosed are methods for obtaining blue anthocyanin pigments and compositions including such pigments. This blue anthocyanin is present at an acidic pH (where anthocyanins are most stable) and may be used as a natural color additive. The methods can include cultivating a plant callus from a plant capable of producing anthocyanin in which the callus is treated with a blue anthocyanin-generating agent at a concentration sufficient to generate callus with blue anthocyanin pigments. The method can also include recovering the blue anthocyanin pigments from the culture. In an example, the blue anthocyanin-generating agent is ammonium molybdate.

US Patent:

57508704, May 12, 1998

Filed:

Feb 3, 1995

Appl. No.:

8/384556

Inventors:

Helena V. Mathews - Portland OR
Richard Keith Bestwick - Portland OR
Adolph J. Ferro - Lake Oswego OR

Assignee:

Agritope, Inc. - Beaverton OR

International Classification:

A01H 500
A01H 508
C12N 1582

US Classification:

800205

Abstract:

An efficient transformation system for plants has been developed that yields high transformation efficiencies and pure transgenic plants. Genomic integration of transgenes was confirmed by genomic DNA hybridization analysis. Pure transgenic plants have been successfully established in soil.

US Patent:

61271829, Oct 3, 2000

Filed:

Oct 23, 1998

Appl. No.:

9/177758

Inventors:

Venkata R. Bommineni - Tigard OR
Helena V. Mathews - Portland OR

Assignee:

Agritope, Inc. - Portland OR

International Classification:

C12N 504
A01H 500

US Classification:

435430

Abstract:

An efficient micropropagation system for fruit-bearing trees has been developed for rapid clonal multiplication of large numbers of shoots within a short time. The micropropagation system provides for transformation and vegetative propagation of fruit-bearing tree shoots to produce uniform populations of transgenic fruit-bearing trees.

US Patent:

60546352, Apr 25, 2000

Filed:

Dec 27, 1996

Appl. No.:

8/777147

Inventors:

Richard K. Bestwick - Portland OR
Jill A. Kellogg - Portland OR
Helena V. Mathews - Portland OR

Assignee:

Agritope, Inc. - Portland OR

International Classification:

C12N 1529
C12N 504
C12N 1582
A01H 500

US Classification:

800278

Abstract:

The present invention is directed to the identification and isolation of a promoter region from a raspberry genome. The promoter is operably linked, in a native raspberry genome, to the coding region of a raspberry E4 gene. The raspberry E4 gene promoter of the invention is capable of regulating moderate level, constitutive expression of a heterologous plant gene under its control. The invention is further directed to chimeric genes, cassette vectors, kits, transgenic plants, and methods employing a raspberry E4 promoter.

US Patent:

20090007298, Jan 1, 2009

Filed:

Jan 3, 2007

Appl. No.:

12/160066

Inventors:

Helena V. Mathews - Portland OR, US
Vaka S. Reddy - Beaverton OR, US
Allan H. Lammers - Portland OR, US

International Classification:

C12N 15/82
C12Q 1/68
C12N 5/04
C12N 15/00
A01H 5/00

US Classification:

800287, 435 6, 435419, 4353201, 800298

Abstract:

Reported herein are methods for obtaining stably transformed callus in ‘Hicksii’, including particularly methods that involve using needles, stem, or bark peel as explant material for transformation. Also provided are descriptions of several promoter activities in directing reporter gene expression in cells, in particular cells in suspension cultures, callus and needles. Transgenic plants (e.g., plants), plant cells, cell lines, and tissues (including seeds) are also provided, in particular those that express one or more enzymes in a paclitaxel biosynthesis pathway.