Ho Thanh Tang

US Patent:

20190376954, Dec 12, 2019

Filed:

Jan 15, 2019

Appl. No.:

16/248157

Inventors:

- Baltimore MD, US
Ho Lam Tang - Baltimore MD, US

International Classification:

G01N 33/50
A01K 67/027

Abstract:

The present invention relates to the field of anastasis, i.e., the process of reversal of apoptosis. More specifically, the present invention provides methods and compositions useful for studying anastasis. In one embodiment, the present invention provides an in vivo biosensor comprising (a) a transcription factor complex comprising the Gal4 transcription factor linked to an enzyme cleavable linker, wherein the transcription factor complex is tethered to the plasma membrane via a transmembrane domain; and (b) a reporter system comprising (1) a first nucleic acid encoding flippase operably linked to the upstream activating sequence that binds Gal4; and (2) a second nucleic acid comprising an FRT-flanked stop codon cassette separating a constitutive promoter and a fluorescent protein open reading frame.

US Patent:

20150026834, Jan 22, 2015

Filed:

Mar 7, 2013

Appl. No.:

14/383156

Inventors:

- Baltimore MD, US
Ho Lam Tang - Baltimore MD, US

International Classification:

G01N 33/50
A01K 67/027

US Classification:

800 18, 424 91

Abstract:

The present invention relates to the field of anastasis, i.e., the process of reversal of apoptosis. More specifically, the present invention provides methods and compositions useful for studying anastasis. In one embodiment, the present invention provides an in vivo biosensor comprising (a) a transcription factor complex comprising the Gal4 transcription factor linked to an enzyme cleavable linker, wherein the transcription factor complex is tethered to the plasma membrane via a transmembrane domain; and (b) a reporter system comprising (1) a first nucleic acid encoding flippase operably linked to the upstream activating sequence that binds Gal4; and (2) a second nucleic acid comprising an FRT-flanked stop codon cassette separating a constitutive promoter and a fluorescent protein open reading frame.