Hongyi Wang

US Patent:

8632975, Jan 21, 2014

Filed:

May 28, 2010

Appl. No.:

12/790760

Inventors:

Peter B. Vander Horn - Encinitas CA, US
Cheng-Yao Chen - Carlsbad CA, US
Guobin Luo - Oceanside CA, US
Michael Previte - Carlsbad CA, US
Jamshid Temirov - Germantown TN, US
Theo Nikiforov - Carlsbad CA, US
Zhaohui Zhou - San Ramon CA, US
Hongye Sun - Belmont CA, US
Yufang Wang - San Carlos CA, US
Stefanie Yukiko Nishimura - Mountain View CA, US
Hongyi Wang - Pearland TX, US
Marian Peris - Belmont CA, US
Barnett B. Rosenblum - San Jose CA, US
Michael Phelan - Hayward CA, US

Assignee:

Life Technologies Corporation - Carlsbad CA

International Classification:

C12Q 1/68

US Classification:

435 612, 435188

Abstract:

Provided herein are compositions and systems for use in polymerase-dependent, nucleotide transient-binding methods. The methods are useful for deducing the sequence of a template nucleic acid molecule and single nucleotide polymorphism (SNP) analyses. The methods rely on the fact that the polymerase transient-binding time for a complementary nucleotide is longer compared to that of a non-complementary nucleotide. The labeled nucleotides transiently-binds the polymerase in a template-dependent manner, but does not incorporate. The methods are conducted under any reaction condition that permits transient binding of a complementary or non-complementary nucleotide to a polymerase, and inhibits nucleotide incorporation.

US Patent:

20080076189, Mar 27, 2008

Filed:

Mar 30, 2007

Appl. No.:

11/694605

Inventors:

Yuri Belosludtsev - The Woodlands TX, US
Mistu Reddy - Pearland TX, US
Anelia Kraltcheva - Houston TX, US
Susan Hardin - College Station TX, US
Tommie Lincecum - Houston TX, US
Hongyi Wang - Houston TX, US
Norha Deluge - Houston TX, US
Uma Nagaswamy - Houston TX, US
Benjamin Stevens - Houston TX, US
Kristi Kincaid - Houston TX, US

Assignee:

VISIGEN BIOTECHNOLOGIES, INC. - Houston TX

International Classification:

G01N 21/64

US Classification:

436172000

Abstract:

Support surfaces are disclosed that are designed to support molecules or molecular assemblies immobilized thereon so that the molecules or molecular assemblies can be observed in single molecule detections systems, where the support surfaces have reduced background and the fluorescent labels associated with the immobilized molecules or molecular assemblies have longer active lifetimes prior to permanent photo-bleaching or deactivation and have improve fluorescence properties and where the surfaces have more uniform fluorescent properties.

US Patent:

20080091005, Apr 17, 2008

Filed:

Jul 20, 2007

Appl. No.:

11/781160

Inventors:

Hongyi Wang - Houston TX, US
Xiaolian Gao - Houston TX, US
Peilin Yu - Houston TX, US
Mitsu Reddy - Pearland TX, US
Susan Hardin - College Station TX, US
Tommie Lincecum - Houston TX, US
Amy Williams - Houston TX, US
Norha Deluge - Houston TX, US
Yuri Belosludtsev - The weedlands TX, US
Steven Menchen - Fremont CA, US
Joe Lam - Castro Valley CA, US

Assignee:

VISIGEN BIOTECHNOLOGIES, INC. - Houston TX
APPLIED BIOSYSTEMS - Foster City CA

International Classification:

C07H 21/04

US Classification:

536026600

Abstract:

Modified nucleotides are disclosed for use in single molecule sequencing, methods for making the modified nucleotides and method for using the modified nucleotides. Linkers for making the modified nucleotide are also disclosed.

US Patent:

20090186343, Jul 23, 2009

Filed:

Feb 21, 2008

Appl. No.:

12/035352

Inventors:

Hongyi Wang - Houston TX, US
Norha Deluge - Pearland TX, US
Kristi Kincaid - Houston TX, US
Yuri Belosludtsev - The Woodlands TX, US
Tommie Lincecum, JR. - Houston TX, US
Amy Williams - Houston TX, US
Amy Bryant - League City TX, US
Ming Fa - Pearland TX, US
Benjamin Stevens - Houston TX, US
Susan H. Hardin - College Station TX, US

Assignee:

VISIGEN BIOTECHNOLOGIES, INC. - Houston TX

International Classification:

C12Q 1/68
C12P 19/34

US Classification:

435 6, 536124

Abstract:

A novel and efficient single pot synthetic schemes are disclosed for preparing modified nucleotides, nucleotide analogs, nucleotide polyphosphates, and nucleotide polyphosphate analogs. The novel method is used to prepare nucleotides, nucleotide analogs, nucleotide polyphosphates, and nucleotide polyphosphate analogs having non-persistent or persistent and non-persistent modifications. Novel biomolecular reactions are also disclosed using the novel modified biomolecules disclosed herein.

US Patent:

20100317005, Dec 16, 2010

Filed:

Mar 15, 2010

Appl. No.:

12/724392

Inventors:

Susan H. Hardin - College Station TX, US
Hongyi Wang - Pearland TX, US
Brent A. Mulder - Sugarland TX, US
Nathan K. Agnew - Richmond TX, US

Assignee:

LIFE TECHNOLOGIES CORPORATION - Carlsbad CA

International Classification:

C12Q 1/68

US Classification:

435 6

Abstract:

Labeled nucleotide triphosphates are disclosed having a label bonded to the gamma phosphate of the nucleotide triphosphate. Methods for using the gamma phosphate labeled nucleotide are also disclosed where the gamma phosphate labeled nucleotide are used to attach the labeled gamma phosphate in a catalyzed (enzyme or man-made catalyst) reaction to a target biomolecule or to exchange a phosphate on a target biomolecule with a labeled gamme phosphate. Preferred target biomolecules are DNAs, RNAs, DNA/RNAs, PNA, polypeptide (e.g., proteins enzymes, protein, assemblages, etc.), sugars and polysaccharides or mixed biomolecules having two or more of DNAs, RNAs, DNA/RNAs, polypeptide, sugars and polysaccharides moieties.

US Patent:

20100323110, Dec 23, 2010

Filed:

Aug 6, 2010

Appl. No.:

12/852356

Inventors:

Yuri BELOSLUDTSEV - The Woodlands TX, US
Nasanshargal BATTULGA - Ulaanbaatar, MN
Mitsu REDDY - Fremont CA, US
Anelia KRALTCHEVA - Carlsbad CA, US
Susan H. HARDIN - College Station TX, US
Hongyi WANG - Pearland TX, US
Norha DELUGE - Pearland TX, US
Uma NAGASWAMY - Pearland TX, US
Benjamin C. STEVENS - Philadelphia PA, US
Kristi K. KINCAID - Houston TX, US

Assignee:

LIFE TECHNOLOGIES CORPORATION - Carlsbad CA

International Classification:

B05D 3/10

US Classification:

427299

Abstract:

Support surfaces are disclosed that are designed to support molecules or molecular assemblies immobilized thereon so that the molecules or molecular assemblies can be observed in single molecule detections systems, where the support surfaces have reduced background and the fluorescent labels associated with the immobilized molecules or molecular assemblies have longer active lifetimes prior to permanent photo-bleaching or deactivation and have improve fluorescence properties and where the surfaces have more uniform fluorescent properties.

US Patent:

20110165652, Jul 7, 2011

Filed:

Jan 14, 2009

Appl. No.:

12/812952

Inventors:

Susan H. Hardin - College Station TX, US
Norha Deluge - Pearland TX, US
Hongyi Wang - Pearland TX, US
Yuri Belosludtsev - The Woodlands TX, US
Kristi Kincaid - Houston TX, US
Anelia Kraltcheva - Houston TX, US
Benjamin Stevens - Philadelphia PA, US
Ming Fa - Pearland TX, US
Amy Bryant - Fredericksburg TX, US
Amy Castillo - Houston TX, US
Hye Eun Kim - Fort Worth TX, US
Uma Nagaswamy - Pearland TX, US
Mitsu Sreedhar Reddy - Pearland TX, US
Alok N. Bandekar - Pearland TX, US
Ivan Pan - Houston TX, US
Andrei Volkov - Houston TX, US

Assignee:

LIFE TECHNOLOGIES CORPORATION - Carlsbad CA

International Classification:

C12N 9/12

US Classification:

435194

Abstract:

Compositions, systems and methods of sequencing are disclosed, where the compositions and systems include polymerase enzymes that have been genetically modified to more efficiently incorporate nucleotides including labels having a detectable properties that are released during incorporation, to augment a rate of labeled nucleotide incorporation, to augment a rate of pyrophosphate release, or to augment two or more of these properties and rates. Also disclosed are terminally labeled and dual labeled nucleotides, and click-chemistry based methods of synthesizing the same.

US Patent:

20200231948, Jul 23, 2020

Filed:

Mar 20, 2020

Appl. No.:

16/825788

Inventors:

- Carlsbad CA, US
Cheng-Yao CHEN - Eugene OR, US
Guobin LUO - Oceanside CA, US
Michael PREVITE - Carlsbad CA, US
Jamshid TEMIROV - Germantown TN, US
Theo NIKIFOROV - Carlsbad CA, US
Zhaohui ZHOU - San Ramon CA, US
Hongye SUN - Belmont CA, US
Yufang WANG - San Carlos CA, US
Stefanie Yukiko NISHIMURA - Mountain View CA, US
Hongyi WANG - Pearland TX, US
Marian PERIS - Dublin CA, US
Barnett ROSENBLUM - San Jose CA, US
Michael PHELAN - Millbrae CA, US

International Classification:

C12N 9/12
C12Q 1/6827
C12Q 1/6869
C12Q 1/6872
C12Q 1/6804
C12Q 1/6818
C12Q 1/68

Abstract:

Provided herein are compositions and systems for use in polymerase-dependent, nucleotide transient-binding methods. The methods are useful for deducing the sequence of a template nucleic acid molecule and single nucleotide polymorphism (SNP) analyses. The methods rely on the fact that the polymerase transient-binding time for a complementary nucleotide is longer compared to that of a non-complementary nucleotide. The labeled nucleotides transiently-binds the polymerase in a template-dependent manner, but does not incorporate. The methods are conducted under any reaction condition that permits transient binding of a complementary or non-complementary nucleotide to a polymerase, and inhibits nucleotide incorporation.