Horace Robert Horton

US Patent:

42845530, Aug 18, 1981

Filed:

Jun 20, 1980

Appl. No.:

6/161645

Inventors:

Rodney J. Brown - Logan UT
Harold E. Swaisgood - Raleigh NC
Horace R. Horton - Raleigh NC

Assignee:

North Carolina State University at Raleigh - Raleigh NC

International Classification:

C09C 308
C09C 310
C09C 312
C12N 996

US Classification:

260112R

Abstract:

A method for the reversible immobilization of proteins through covalent attachment to inorganic oxide surfaces, such as glass or silica, via thioester containing coupling chains. Resulting succinamidopropyl-surfaces are converted into the acyl derivative using anhydrous thionyl chloride, and then treated with either 3-mercaptopropionic acid or mercaptoacetic acid. Thus derivatized, the material is dried, and can be stored for extended periods of time without detectable loss of reactivity with proteins. Immobilization of proteins can be readily achieved simply by suspending the derivatized material in buffer (pH=5 to 8) and recycling the protein solution through the material. Following immobilization, protein molecules can be released from the surface by cleavage of the coupling chains at the thioester linkage through treatment with hydroxylamine.