Howard F Sands

US Patent:

7807416, Oct 5, 2010

Filed:

Oct 15, 2008

Appl. No.:

12/251889

Inventors:

Luigi Grasso - Bryn Mawr PA, US
Nicholas C. Nicolaides - Garnett Valley PA, US
Howard Sands - Wilmington DE, US
Philip M. Sass - Audubon PA, US

Assignee:

Morphotek, Inc. - Exton PA

International Classification:

C12P 21/08

US Classification:

435 7021, 435 691, 435 701, 435 711, 435326, 435328, 5303873, 5303881

Abstract:

Dominant negative alleles of human mismatch repair genes can be used to generate hypermutable cells and organisms. By introducing these genes into cells and transgenic animals, new cell lines and animal varieties with novel and useful properties can be prepared more efficiently than by relying on the natural rate of mutation. These methods are useful for generating genetic diversity within immunoglobulin genes directed against an antigen of interest to produce altered antibodies with enhanced biochemical activity. Moreover, these methods are useful for generating antibody-producing cells with increased level of antibody production. The invention also provides methods for increasing the effector function of monoclonal antibodies and monoclonal antibodies with increased effector function.

US Patent:

20050054048, Mar 10, 2005

Filed:

Jul 29, 2004

Appl. No.:

10/901650

Inventors:

Luigi Grasso - Bala Cynwyd PA, US
Nicholas Nicolaides - Boothwyn PA, US
Howard Sands - Wilmington DE, US
Philip Sass - Audubon PA, US

International Classification:

C07H021/04
C07K016/18
C12N015/87

US Classification:

435069100, 435455000, 435326000, 530387100, 536023530, 800006000

Abstract:

Dominant negative alleles of human mismatch repair genes can be used to generate hypermutable cells and organisms. By introducing these genes into cells and transgenic animals, new cell lines and animal varieties with novel and useful properties can be prepared more efficiently than by relying on the natural rate of mutation. These methods are useful for generating genetic diversity within immunoglobulin genes directed against an antigen of interest to produce altered antibodies with enhanced biochemical activity. Moreover, these methods are useful for generating antibody-producing cells with increased level of antibody production. The invention also provides methods for increasing the effector function of monoclonal antibodies and monoclonal antibodies with increased effector function.