Hui Zong

US Patent:

20050125850, Jun 9, 2005

Filed:

Dec 3, 2003

Appl. No.:

10/727399

Inventors:

Liqun Luo - Stanford CA, US
Hui Zong - Stanford CA, US

International Classification:

A01K067/027
C12N015/85

US Classification:

800018000, 435455000

Abstract:

Recombination in mammalian somatic cell chromosomes is promoted and marked by a method called mosaic analysis with double marker (MADM). Mouse “knock-in” techniques are used to create pairs of chromosomes in which recombinase target sites are placed at homologous chromosomal locations. The knock-in constructs are engineered so that cellular markers, such as green or red fluorescent protein (GFP or RFP), are only expressed after recombinase-induced recombination. This system provides high-sensitivity detection of recombinase-induced mitotic recombination, even down to the single cell level. When this recombination is induced in a mouse heterozygous for a mutation in a gene distal to the “knock-in” locus on the same chromosome, it results in homozygosity of this mutation in the labeled cells. This allows the analysis in singly-labeled neurons of genes whose pleiotropic effects might otherwise result in early lethality.

US Patent:

20110035815, Feb 10, 2011

Filed:

Aug 9, 2010

Appl. No.:

12/806302

Inventors:

Hui Zong - Eugene OR, US
Chong Liu - Eugene OR, US

International Classification:

G01N 33/48
A01K 67/027
C12Q 1/02
C12Q 1/68

US Classification:

800 3, 800 18, 435 29, 435 6

Abstract:

Disclosed herein are transgenic mouse tumor models. A portion of the cells of the disclosed transgenic mice undergo directed somatic recombination and the mouse is therefore a mosaic for homozygous knockout of p53, NF1, or both p53 and NF1 genes. The homozygous null cells resulting from the somatic recombination also express a detectable fluorescent protein, and the resulting sibling cells, which are wild type for p53, NF1, or both p53 and NF1, express a different detectable fluorescent protein. Also disclosed herein are methods of identifying compounds for treating or preventing a tumor, using the disclosed transgenic mice.

US Patent:

20120124686, May 17, 2012

Filed:

Nov 10, 2011

Appl. No.:

13/293890

Inventors:

Liqun Luo - Palo Alto CA, US
Ruby Yanru Tsai - San Jose CA, US
Bosiljka Tasic - Palo Alto CA, US
Simon Hippenmeyer - Menlo Park CA, US
Hui Zong - Eugene OR, US

International Classification:

A01K 67/027
C12N 15/87

US Classification:

800 18, 435462

Abstract:

The present disclosure provides a method of making a mammal (e.g., a rodent, such as a mouse) by integrating an intact polynucleotide sequence into a specific genomic locus of the mammal to result in a transgenic mammal. A transgenic mammal made by the methods of the present disclosure would contain a known copy number (e.g., one) of the inserted polynucleotide sequence at a predetermined location. The method involves introducing a site-specific recombinase and a targeting construct, containing a first recombination site and the polynucleotide sequence of interest, into the mammalian cell. The genome of the cell contains a second recombination site and recombination between the first and second recombination sites is facilitated by the site-specific, uni-directional recombinase. The result of the recombination is site-specific integration of the polynucleotide sequence of interest in the genome of the mammal. This inserted sequence is then also transmitted to the progeny of the mammal.