Itai Benhar

US Patent:

58891579, Mar 30, 1999

Filed:

Oct 28, 1994

Appl. No.:

8/331396

Inventors:

Ira Pastan - Potomac MD
Itai Benhar - Rockville MD
Eduardo A. Padlan - Kensington MD
Sun-Hee Jung - Rockville MD
Byungkook Lee - Potomac MD

Assignee:

The United States of America as represented by the Department of Health
and Human Services - Washington DC

International Classification:

C07K 1600
A61K 39395

US Classification:

5303871

Abstract:

This invention provides for recombinant single chain antibodies capable of specifically binding to a Lewis. sup. Y -related carbohydrate antigen and fusion proteins comprising these antibodies. More particularly, the invention provides for humanized chain Fv regions of the monoclonal antibodies B1, B3 and B5 and fusion proteins incorporating these humanized antibodies. The antibodies may comprise humanized variable heavy (V. sub. H) chains, humanized variable light (V. sub. L) chains, or both. The invention also provides for DNA sequences encoding the various humanized antibodies. In addition, the invention provides for methods of detecting cells bearing a Lewis. sup. Y antigen in a patient and for methods of killing or inhibiting the growth of cells bearing a Lewis. sup. Y antigen in a patient.

US Patent:

59817267, Nov 9, 1999

Filed:

Oct 28, 1994

Appl. No.:

8/331397

Inventors:

Ira Pastan - Potomac MD
Itai Benhar - Rockville MD

Assignee:

The United States of America as represented by the Department of Health
and Human Services - Washington DC

International Classification:

A61K 39395
C07M 2104

US Classification:

536 2353

Abstract:

This invention provides for recombinant single chain antibodies capable of specifically binding to a Lewis. sup. Y -related carbohydrate antigen and fusion proteins comprising these antibodies. More particularly, the invention provides for single chain Fv regions of the monoclonal antibodies B1 and B5. The invention also provides for a number of stabilizing mutations of the Lewis. sup. Y -binding monoclonal antibody B3. In addition, the invention provides for methods of detecting cells bearing a Lewis. sup. Y antigen in a patient and for methods of killing or inhibiting the growth of cells bearing a Lewis. sup. Y antigen in a patient.

US Patent:

62875620, Sep 11, 2001

Filed:

Jan 8, 1999

Appl. No.:

9/227693

Inventors:

Ira Pastan - Potomac MD
Itai Benhar - Rockville MD
Eduardo A. Padlan - Kensington MD
Sun-Hee Jung - Rockville MD
Byungkook Lee - Potomac MD

Assignee:

The United States of America as represented by the Department of Health and
Human Services - Washington DC

International Classification:

A61K 39395

US Classification:

4241831

Abstract:

The invention provides methods for inhibiting the growth of a cell bearing a Lewis. sup. Y antigen. The methods involve contacting the cell with a composition comprising an Fv region of a light chain of a monoclonal antibody selected from B1, B3, and B5, and the Fv region of a humanized heavy chain of a monoclonal antibody independently selected from B1, B3, and B5, provided that if the heavy and the light chains are from the same antibody, the residue at position 95 of the heavy chain is a serine, and, if the antibody chain is from B3, the residue at position 4 of the light chain can be a leucine and the residue at position 82b of the heavy chain can be an arginine. The Fv regions are joined to an effector molecule selected from a chemotherapeutic agent, a toxin, a radioisotope, and a liposome loaded with a chemotherapeutic agent. In preferred embodiments, the heavy chain and the light chain are recombinantly fused, and the Fv regions are recombinantly fused to a toxin.