James M Kadushin

US Patent:

7888018, Feb 15, 2011

Filed:

Mar 6, 2007

Appl. No.:

11/714989

Inventors:

Robert C. Getts - Collegeville PA, US
Kelly Sensinger - Perkasie PA, US
James Kadushin - Gilbertsville PA, US

Assignee:

Genisphere, LLC - Hatfield PA

International Classification:

C12Q 1/68
C12P 19/34

US Classification:

435 6, 435 911

Abstract:

Compositions and methods are provided for amplifying nucleic acid molecules. The nucleic acid molecules can be used in various research and diagnostic applications, such as gene expression studies involving nucleic acid microarrays.

US Patent:

8097418, Jan 17, 2012

Filed:

Mar 31, 2009

Appl. No.:

12/415459

Inventors:

Robert C. Getts - Collegeville PA, US
Kelly Sensinger - Perkasie PA, US
James Kadushin - Gilbertsville PA, US

Assignee:

Genisphere, LLC - Hatfield PA

International Classification:

C12Q 1/68
C12P 19/34

US Classification:

435 612, 435 912, 435 9121, 435 913

Abstract:

Methods and kits are provided for performing multiple rounds of sense RNA synthesis. The sense RNA molecules can be used in various research and diagnostic applications, such as gene expression studies involving nucleic acid microarrays.

US Patent:

8343721, Jan 1, 2013

Filed:

Jan 25, 2011

Appl. No.:

13/013262

Inventors:

Robert C. Getts - Collegeville PA, US
Kelly Sensinger - Perkasie PA, US
James Kadushin - Gilbertsville PA, US

Assignee:

Genisphere, LLC - Hatfield PA

International Classification:

C12Q 1/68
C12P 19/34

US Classification:

435 61, 435 911

Abstract:

Compositions and methods are provided for amplifying nucleic acid molecules. The nucleic acid molecules can be used in various research and diagnostic applications, such as gene expression studies involving nucleic acid microarrays.

US Patent:

8617817, Dec 31, 2013

Filed:

Feb 11, 2011

Appl. No.:

13/025585

Inventors:

Robert C. Getts - Collegeville PA, US
James Kadushin - Gilbertsville PA, US

Assignee:

Genisphere, LLC - Hatfield PA

International Classification:

C12Q 1/68
C07H 21/04
C12P 19/34

US Classification:

435 612, 536 23, 536 24, 435 912

Abstract:

Methods for preparing strand-specific sequencing libraries of oligonucleotides using an RNA polymerase promoter to re-transcribe antisense cDNA which has been reverse transcribed from mRNA are provided. The transcription step linearly amplifies sRNA prior to production of double-stranded cDNA to be sequenced and may be sufficient to eliminate the conventional PCR amplification step prior to sequencing. The methods incorporate anchor sequences, amplification sequences and other sequences required for a particular sequencing system or reaction by hybridization and extension of primers, and transcription of RNA, rather than ligation, thus reducing the number of steps and the time required for sample preparation for sequencing of RNA. Use of primer hybridization and transcription reactions in the methods also results in a library that exhibits reduced 3′ sequence bias.

US Patent:

20020072060, Jun 13, 2002

Filed:

Jul 19, 2001

Appl. No.:

09/908950

Inventors:

Robert Getts - Collegeville PA, US
James Kadushin - Gilbertsville PA, US

International Classification:

C12Q001/68
C12P019/34

US Classification:

435/006000, 435/091200

Abstract:

The present invention is directed to a method for determining the presence of a specific nucleotide sequence in an RNA reagent of a target sample utilizing a capture reagent having at least one first arm containing a label capable of emitting a detectable signal and at least one second arm having a nucleotide sequence complementary to a capture sequence attached to the RNA reagent on a microarray.

US Patent:

20020094538, Jul 18, 2002

Filed:

Jan 14, 2002

Appl. No.:

10/050088

Inventors:

Robert Getts - Collegeville PA, US
James Kadushin - Gilbertsville PA, US

International Classification:

C12Q001/68

US Classification:

435/006000

Abstract:

The present invention is directed to a method for determining the presence of a specific nucleotide sequence in a cDNA reagent of a target sample utilizing a capture reagent having at least one first arm containing a label capable of emitting a detectable signal and at least one second arm having a nucleotide sequence complementary to a capture sequence attached to the cDNA reagent on a microarray through temperature cycling.

US Patent:

20040009487, Jan 15, 2004

Filed:

Sep 3, 2002

Appl. No.:

10/234069

Inventors:

James Kadushin - Gilbertsville PA, US
Robert Getts - Collegeville PA, US

International Classification:

C12Q001/68
C07H021/04

US Classification:

435/006000, 536/024300

Abstract:

Methods are provided for blocking non-specific and specific hybridization of nucleic acid samples on a microarray. The method of the present invention comprises applying a blocking reagent to the microarray, wherein the blocking reagent comprises modified nucleotide bases, preferably LNA (Locked Nucleic Acid—modified bicyclic monomeric units with a 2′-O-4′-C methylene bridge). In further embodiments, the method includes applying a mixture including: a) a cDNA reagent obtained from mRNA of a target sample, the cDNA having a capture sequence; b) a dendrimer with a label for emitting a detectable signal and a second nucleotide sequence complementary to the capture sequence; and c) an blocking reagent containing LNA to a microarray, for producing a detectable signal from said label whereby a hybridization pattern is generated on the microarray.

US Patent:

20040110196, Jun 10, 2004

Filed:

Aug 19, 2003

Appl. No.:

10/643322

Inventors:

James Kadushin - Gilbertsville PA, US
Robert Getts - Collegeville PA, US

International Classification:

C12Q001/68
G01N033/53
C12M001/34

US Classification:

435/006000, 435/007100, 435/287200

Abstract:

A composition for forming a protective coating over the surface of an assay substrate having an indicating agent capable of generating a detectable signal associated therewith, includes a protective coating forming material, and a delivery system for delivering the protective coating forming material in an amount sufficient to coat the surface of the assay substrate, wherein the delivery system evaporates from the surface of the assay substrate to form a protective coating at least substantially composed of the protective coating forming material that is at least substantially transparent to the detectable signal generated by the indicating agent. The present invention is further directed to a method for forming the protective coating over the surface of the assay substrate, and a substrate produced by such method.