James Louis Sherley

US Patent:

7645610, Jan 12, 2010

Filed:

Feb 18, 2003

Appl. No.:

10/369428

Inventors:

James L. Sherley - Boston MA, US
Hsuan-Shu Lee - Taipei, TW
Gracy G. Crane - Allston MA, US

Assignee:

Massachusetts Institute of Technology - Cambridge MA

International Classification:

C12N 5/08

US Classification:

435370, 435375

Abstract:

The present invention is directed to methods for readily generating hepatocyte precursor cell lines that retain hepatocyte-specific functions after extensive in vitro culturing. The methods comprise isolating and culturing hepatocyte precursor cell lines under permissive culture conditions that suppress asymmetric cell kinetics and allow exponential growth of the precursor cells, followed by transferring the hepatocyte precursor cell lines to non-permissive culture conditions that allow expression of asymmetric cell kinetics and induce expression of hepatocyte-specific characteristics.

US Patent:

7655465, Feb 2, 2010

Filed:

Jun 7, 2005

Appl. No.:

11/147013

Inventors:

James L. Sherley - Boston MA, US
Johnathan King - Plano TX, US

Assignee:

Massachusetts Institute of Technology - Cambridge MA

International Classification:

C12N 5/06

US Classification:

435377, 435325, 435375, 435378, 51426334

Abstract:

The present invention is directed to methods for readily propagating somatic hair follicle stem cells or melanocyte stem cells. The methods comprise enhancing guanine nucleotide (GNP) biosynthesis, thereby expanding guanine nucleotide pools. This in turn conditionally suppresses asymmetric cell kinetics in the explanted cells. The methods of the invention include pharmacological methods and genetic methods. For example, the resulting cultured somatic hair follicle stem cells can be used for a variety of applications including cell replacement therapies such as hair transplants, gene therapies, and tissue engineering applications, such as the generation of artificial skin and skin regeneration strategies including skin grafts.

US Patent:

7867712, Jan 11, 2011

Filed:

Aug 8, 2006

Appl. No.:

12/063182

Inventors:

James L. Sherley - Boston MA, US
Min-Soo Noh - Seoul, KR

Assignee:

Massachusetts Institute of Technology - Cambridge MA

International Classification:

C12Q 1/68
G01N 33/53
C12P 19/34

US Classification:

435 6, 435 71, 435 912

Abstract:

The present invention is directed to nucleic acid sequences whose expression is associated with different cell states, including nucleic acid sequences whose expression is induced at least 100-fold, or alternatively upregulated, in cells exhibiting asymmetric self-renewal relative to other cells. The invention is also directed to nucleic acid sequences whose expression is induced at least 100-fold, or alternatively upregulated, in cells exhibiting symmetric self-renewal relative to other cells.

US Patent:

7883891, Feb 8, 2011

Filed:

Jul 17, 2002

Appl. No.:

10/483601

Inventors:

James L Sherley - Boston MA, US
Joshua R. Merok - Chicago IL, US

Assignee:

Massachusetts Institute of Technology - Cambridge MA

International Classification:

C12N 5/08

US Classification:

435366

Abstract:

The present invention is directed to unique properties of stem cells, including methods to identify stem cell markers by identifying molecules associated specifically with chromosomes in stem cells. More particularly, we have discovered that somatic stem cells repeatedly inherit an entire complement of chromosomes that contain the same parental template DNA strands from one generation to the next. The present invention also provides methods related to diagnosis, prognosis, and treatments for cancer and aging in mammalian tissus, including human.

US Patent:

8404481, Mar 26, 2013

Filed:

Nov 16, 2009

Appl. No.:

12/619107

Inventors:

James L. Sherley - Boston MA, US
Hsuan-Shu Lee - Taipei, TW
Gracy G. Crane - Allston MA, US

Assignee:

Massachusetts Institute of Technology - Cambridge WA

International Classification:

C12N 5/00
C12N 5/02
C12N 5/07
C12N 5/071

US Classification:

435370, 435375, 435377

Abstract:

The present invention is directed to methods for readily generating hepatocyte precursor cell lines that retain hepatocyte-specific functions after extensive in vitro culturing. The methods comprise isolating and culturing hepatocyte precursor cell lines under permissive culture conditions that suppress asymmetric cell kinetics and allow exponential growth of the precursor cells, followed by transferring the hepatocyte precursor cell lines to non-permissive culture conditions that allow expression of asymmetric cell kinetics and induce expression of hepatocyte-specific characteristics.

US Patent:

20030133918, Jul 17, 2003

Filed:

Jul 10, 2002

Appl. No.:

10/192110

Inventors:

James Sherley - Boston MA, US

International Classification:

A61K048/00
A61K031/522
C12N005/08
C12N015/85
A61K031/7076

US Classification:

424/093210, 435/455000, 435/366000, 514/045000, 514/263340

Abstract:

The present invention is directed to methods for readily propagating somatic tissue stem cells ex vivo. The methods comprise enhancing guanine nucleotide (GNP) biosynthesis, thereby expanding guanine nucleotide pools. This in turn conditionally suppresses asymmetric cell kinetics in the explanted tissue cells. The methods of the invention include pharmacological methods and genetic methods. The resulting cultured somatic stem cells can be used for a variety of applications including cell replacement therapies such as bone marrow transplants, gene therapies, tissue engineering, and in vitro organogenesis.

US Patent:

20120295351, Nov 22, 2012

Filed:

Dec 3, 2010

Appl. No.:

13/513612

Inventors:

James L. Sherley - Boston MA, US

Assignee:

BOSTON BIOMEDICAL RESEARCH INSTITUTE, INC. - Watertown MA

International Classification:

C12N 5/071

US Classification:

435377

Abstract:

Embodied herein are methods of reprogramming somatic cells or tissue stem cells to a more multipotent state or even a pluripotent state, the methods do not involve gene transfer of master transcription factor genes/proteins. The methods are also useful for rapid and efficient cloning of induced pluripotent stem cells after gene transfer of master transcription factor genes/proteins.

US Patent:

20130004965, Jan 3, 2013

Filed:

Dec 3, 2010

Appl. No.:

13/513289

Inventors:

James L. Sherley - Boston MA, US

Assignee:

BOSTON BIOMEDICAL RESEARCH INSTITUTE, INC. - Watertown MA

International Classification:

G01N 33/566
G01N 33/573

US Classification:

435 74, 435 71, 435 79

Abstract:

This invention provides methods of determining the number and percent of tissue specific stem cells (TSSCs) in a sample of cells, a population of cells or a sample of tissue. The methods rely on detecting the pattern-specific asymmetric localization of asymmetric self-renewal associated (ASRA) proteins or cell cycle specific proteins (CSSP) in cell undergoing asymmetrical self-renewal, which is a characteristic of TSSCs. The methods can be applied to any situations in which the percent of TSSC is desired such as laboratory research on adult stem cells, in drug development tests, prognostic indicator and therapeutic index, as a diagnostic and prognostic indicator and in monitoring TSSC expansion, e.g., in cell manufacturing processes.