Janet S Marks

US Patent:

47741775, Sep 27, 1988

Filed:

Jul 15, 1985

Appl. No.:

6/755189

Inventors:

Janet M. Marks - Coronado CA

Assignee:

Vet-Test Partners - San Diego CA

International Classification:

G01N 3353
G01N 33544

US Classification:

435 7

Abstract:

A method for detecting the presence of antibody or antigen in a liquid sample suspected of containing the antibody or antigen. To a test panel comprising a nitrocellulose carrier having stably bonded thereto a microsized spot consisting of either a primary ligand for that antibody or an antibody for that antigen, is added sufficient liquid containing non-specific globulin for the material of the spot, to block adjacent binding sites. Then a small liquid drop of the sample suspected of containing the antibody or antigen is added. After incubation of the panel, in tests for detecting an antibody, a drop of liquid containing labeled antiglobulin, which is an anti-immunoglobulin for that antibody, is added. In tests for detecting an antigen, the specific antibody-enzyme conjugate is added. After further incubation wash solution is added to the spot, and the test panel is assayed for presence of the label.