Janice D Barnes

US Patent:

60279389, Feb 22, 2000

Filed:

Mar 20, 1998

Appl. No.:

9/045291

Inventors:

Allen C. Barnes - Newport Beach CA
Janice S. Barnes - Newport Beach CA

International Classification:

C12N 500
C12N 100
C12C 100
A01N 102

US Classification:

435392

Abstract:

A patient replica is created from a layered culture medium where solid culture medium is formed so that a discontinuity exists between the layers. An infusion port is provided in registration with the discontinuity so that a fresh unadulterated sample of patient blood can be infused into the discontinuity to form a thin layer of blood between the layers of culture medium. The thin layer obviates the requirement for any anticoagulant allowing blood-borne pathogens to be readily cultured without using broth. Further antibiotics or other drug samples may be placed on the surface of the culture medium above the blood layer so that the antibiotic can diffuse through the culture medium and reveal the sensitivities of the cultured pathogens. Other samples of pathogens or tissues can be placed on the surface of the culture medium so that effects of drugs or growth factors present in the patient blood can be observed thereby allowing the entrapped blood layer to act as a biological replica of the patient.

US Patent:

62040561, Mar 20, 2001

Filed:

Sep 3, 1999

Appl. No.:

9/390859

Inventors:

Allen C. Barnes - Newport Beach CA
Janice S. Barnes - Newport Beach CA

International Classification:

C12N 500

US Classification:

435392

Abstract:

A patient replica is created from layered culture medium where solid culture medium is formed so that a discontinuity exists, for example, between layers of culture medium or between culture medium and the container holding the medium. An injection port is provided in registration with the discontinuity so that a sample of patient blood can be infused into the discontinuity to form a thin layer of blood in contact with a surface of culture medium. The thin layer of blood obviates the requirement for any anticoagulant allowing blood-borne pathogens to be readily cultured without using broth. Further antibiotics or other drug samples may be placed on an exposed surface of the culture medium above the blood layer so that the antibiotic can diffuse through the culture medium and reveal the sensitivities of the cultured pathogens. Other samples of pathogens or tissues can be placed on the surface of the culture medium so that effects of drugs or growth factors present in the patient blood can be observed thereby allowing the entrapped blood layer and culture medium, held to natural patient conditions, to act as a biological replica of the patient.