Janice CHEN - New York NY, US Shuangping SHI - Kenilworth NJ, US
Assignee:
Merck Sharp & Dohme Corp. - Rahway NJ
International Classification:
C07K 14/54
Abstract:
The present invention provides a CHO cell that includes a polynucleotide encoding a polypeptide of interest. The CHO cell may include a heterologous polynucleotide encoding IL6 and/or an IL6 pathway member or, in the absence of the heterologous IL6 or an IL6 pathway member polynucleotide, the CHO cell can be cultured in the presence of exogenously added IL6 polypeptide. Such CHO cells exhibit superior expression of the polypeptide of interest in culture. Methods for expressing a polypeptide of interest and methods for making such CHO cells are part of the present invention.
R/Ga
Junior Art Director
Bandujo Advertising + Design Jan 2018 - May 2018
Design Intern
Infor May 2017 - Jul 2017
Design Intern
Havas Health Jun 2016 - Aug 2016
Creative Intern
Education:
Fashion Institute of Technology 2016 - 2018
Bachelors, Bachelor of Fine Arts, Advertising, Design
Fashion Institute of Technology 2014 - 2016
Associates, Associate of Arts, Communication, Design
Arlington High School (New York)
Stony Brook University
Arlington High School
Shanghai Commercial Bank Hk
Operator and Customer Service
China King Restaurant May 2005 - Dec 2006
Waitress
Santa Rosa Business May 2004 - Aug 2004
Assistant Office Administrator
Education:
Stony Brook University 2004 - 2007
Bachelor of Applied Science, Bachelors, Economics, Business, Finance
Skills:
Customer Service Time Management Teamwork Deposits Microsoft Word Microsoft Excel English
Govt. of Singapore Investment Corporation Singapore Aug 2012 to Aug 2013 Associate, Total Return EquitiesYale Department of Political Science New Haven, CT Sep 2009 to Apr 2012 Research Assistant to Deputy Provost, Professor Frances RosenbluthBank of America Merrill Lynch New York, NY Jun 2011 to Aug 2011 Summer Analyst, Global Markets Sales & TradingMAI Wealth Advisors Cleveland, OH Jun 2010 to Aug 2010 Summer Investments Intern, Dividend Strategy & Options Overlay TeamGroppe, Long & Littell Houston, TX Jun 2009 to Aug 2009 Production AnalystYale Department of Geology & Geophysics New Haven, CT Feb 2009 to May 2009 Research Assistant to Professor Jun Korenaga & Dr. Tadashi Kito
Education:
Yale University New Haven, CT 2008 to 2012 Bachelor of Arts in Economics, Distinction
But co-first authors Janice Chen, Enbo Ma and Lucas Harrington in Doudnas lab discovered that when Cas12a binds and cuts a targeted double-stranded DNA sequence, it unexpectedly unleashes indiscriminate cutting of all single-stranded DNA in a test tube.