Janice CHEN - New York NY, US Shuangping SHI - Kenilworth NJ, US
Assignee:
Merck Sharp & Dohme Corp. - Rahway NJ
International Classification:
C07K 14/54
Abstract:
The present invention provides a CHO cell that includes a polynucleotide encoding a polypeptide of interest. The CHO cell may include a heterologous polynucleotide encoding IL6 and/or an IL6 pathway member or, in the absence of the heterologous IL6 or an IL6 pathway member polynucleotide, the CHO cell can be cultured in the presence of exogenously added IL6 polypeptide. Such CHO cells exhibit superior expression of the polypeptide of interest in culture. Methods for expressing a polypeptide of interest and methods for making such CHO cells are part of the present invention.
Medicine Doctors
Dr. Janice Y Chen, Seattle WA - DDS (Doctor of Dental Surgery)
Harrison Design Landscape Architecture Sep 2014 - Apr 2017
Designer
Seattle Parks and Recreation Jun 2013 - Dec 2013
Landscape Design Intern and Student Engineer
Microsoft Jul 2009 - Sep 2009
Research Intern
Education:
University of Washington 2009 - 2014
Bachelor of Liberal Arts, Bachelors, Landscape Architecture
Bellevue Senior High School 2007 - 2009
Skills:
Landscape Architecture Computer Graphics Autocad Urban Design Adobe Creative Suite Photoshop Illustrator Hand Drafting Sketchup Arcgis Indesign Landscape Design Microsoft Office Low Impact Development Sustainable Design Sustainability Site Planning Urban Planning Design Research
Benaroya Research Institute Apr 2011 - Oct 2012
Research Technician Ii
University of Washington Mar 2009 - Apr 2011
Research Technician I
Education:
University of Washington 2011 - 2013
Masters, Bioengineering
Saint Louis University 2008
University of Washington 2002 - 2006
Bachelors, Bachelor of Science, Biochemistry, Biology
Skills:
Cell Culture Qpcr Molecular Biology Pcr Cell Biology Transfection Elisa Flow Cytometry Immunology Microsoft Office Microsoft Excel Powerpoint Tissue Culture Western Blotting Molecular Cloning Fluorescence Microscopy Rna Isolation Microscopy Dna Extraction Confocal Microscopy Microsoft Word
Govt. of Singapore Investment Corporation Singapore Aug 2012 to Aug 2013 Associate, Total Return EquitiesYale Department of Political Science New Haven, CT Sep 2009 to Apr 2012 Research Assistant to Deputy Provost, Professor Frances RosenbluthBank of America Merrill Lynch New York, NY Jun 2011 to Aug 2011 Summer Analyst, Global Markets Sales & TradingMAI Wealth Advisors Cleveland, OH Jun 2010 to Aug 2010 Summer Investments Intern, Dividend Strategy & Options Overlay TeamGroppe, Long & Littell Houston, TX Jun 2009 to Aug 2009 Production AnalystYale Department of Geology & Geophysics New Haven, CT Feb 2009 to May 2009 Research Assistant to Professor Jun Korenaga & Dr. Tadashi Kito
Education:
Yale University New Haven, CT 2008 to 2012 Bachelor of Arts in Economics, Distinction
But co-first authors Janice Chen, Enbo Ma and Lucas Harrington in Doudnas lab discovered that when Cas12a binds and cuts a targeted double-stranded DNA sequence, it unexpectedly unleashes indiscriminate cutting of all single-stranded DNA in a test tube.