Janice A Clements

US Patent:

60602368, May 9, 2000

Filed:

Dec 29, 1998

Appl. No.:

9/222227

Inventors:

Janice E. Clements - Columbia MD
Opendra Narayan - Timonium MD
Susan Vandewoude - Loveland CO
Juergen A. Richt - Moensheim, DE

Assignee:

The Johns Hopkins University School of Medicine - Baltimore MD

International Classification:

C12Q 170

US Classification:

435 5

Abstract:

Borna disease virus (BDV) causes a rare neurological disease in horses and sheep. A subtractive cDNA expression library was constructed with poly A-selected RNA from a BDV infected MDCK cell line. A clone (B8) was isolated that specifically hybridizes to RNA isolated from BDV-infected brain tissue and BDV-infected cell lines. This clone hybridizes to four BDV-specific positive strand RNAs and one negative strand RNA in BDV-infected rat brain. Nucleotide sequence analysis of the clone suggests that it represents a full length mRNA which contains several open reading frames. The Borna Disease Virus DNA sequences as well as proteins encoded by the BDV DNA sequences are provided.

US Patent:

57767459, Jul 7, 1998

Filed:

Oct 11, 1995

Appl. No.:

8/540721

Inventors:

Gary Wayne Ketner - Columbia MD
Philip Andrew Hieter - Baltimore MD
Janice E. Clements - Columbia MD

Assignee:

The Johns Hopkins University - Baltimore MD

International Classification:

C12N 1510
C12N 119

US Classification:

4351723

Abstract:

Homologous recombination techniques are used to produce yeast artificial chromosomes (YAC) containing foreign target DNA by utilizing at least three DNA segments which yield the vector. Preferred embodiments allow the production of viral DNA which is infectious.

US Patent:

56544014, Aug 5, 1997

Filed:

Nov 30, 1994

Appl. No.:

8/351176

Inventors:

Janice E. Clements - Columbia MD
Opendra Narayan - Timonium MD
Susan Vandewoude - Loveland CO
Juergen A. Richt - Moensheim, DE

Assignee:

The Johns Hopkins University - Baltimore MD

International Classification:

C07K 100
C07K 1400
C12P 2106
C12Q 170

US Classification:

530350

Abstract:

Borna disease virus (BDV) causes a rare neurological disease in horses and sheep. A subtractive cDNA expression library was constructed with poly A-selected RNA from a BDV infected MDCK cell line. A clone (B8) was isolated that specifically hybridizes to RNA isolated from BDV-infected brain tissue and BDV-infected cell lines. This clone hybridizes to four BDV-specific positive strand RNAs and one negative strand RNA in BDV-infected rat brain. Nucleotide sequence analysis of the clone suggests that it represents a full length mRNA which contains several open reading frames. The Borna Disease Virus DNA sequences as well as proteins encoded by the BDV DNA sequences are provided.

US Patent:

58544178, Dec 29, 1998

Filed:

May 31, 1995

Appl. No.:

8/455976

Inventors:

Janice Clements - Columbia MD
Opendra Narayan - Timonium MD
Susan Vandewoude - Loveland CO
Juergen Richt - Moensheim, DE

Assignee:

The Johns Hopkins University - Baltimore MD

International Classification:

C07H 2104
C12N 1531

US Classification:

536 2372

Abstract:

Borna disease virus (BDV) causes a rare neurological disease in horses and sheep. A subtractive cDNA expression library was constructed with poly A-selected RNA from a BDV Infected MDCK cell line. A clone (B8) was isolated that specifically hybridizes to RNA isolated from BDV-infected brain tissue and BDV-infected cell lines. This clone hybridizes to four BDV-specific positive strand RNAs and one negative strand RNA in BDV-infected rat brain. Nucleotide sequence analysis of the clone suggests that it represents a full length mRNA which contains several open reading frames. The Borna Disease Virus DNA sequences as well as proteins encoded by the BDV DNA sequences are provided.