Jared Renton Leadbetter

US Patent:

8551725, Oct 8, 2013

Filed:

Jan 24, 2008

Appl. No.:

12/019593

Inventors:

Jared Leadbetter - Altadena CA, US
Jean J. Huang - Pasadena CA, US

Assignee:

California Institute of Technology - Pasadena CA

International Classification:

C12Q 1/34
C12Q 1/68
C40B 10/00
C40B 30/06

US Classification:

435 18, 435 611, 506 2, 506 10

Abstract:

Screening assays that allow for the identification of agents that increase acyl homoserine lactone (AHL) acylase expression and/or AHL acylase activity in γ-proteobacteria such as. Such agents are useful, for example, for inhibiting quorum sensing activity of such bacteria by increasing degradation of long chain, but not short chain, AHLs and, therefore, can be useful for treating infections by such bacteria.

US Patent:

20050053952, Mar 10, 2005

Filed:

Oct 2, 2003

Appl. No.:

10/678946

Inventors:

Jong Hong - Pasadena CA, US
Vincent Studer - Paris, FR
W. Anderson - San Marino CA, US
Stephen Quake - San Marino CA, US
Jared Leadbetter - Altadena CA, US

Assignee:

California Institute of Technology - Pasadena CA

International Classification:

C12Q001/68
C12M001/34

US Classification:

435006000, 435287200

Abstract:

Nucleic acid from cells and viruses sampled from a variety of environments may purified and expressed utilizing microfluidic techniques. In accordance with one embodiment of the present invention, individual or small groups of cells or viruses may be isolated in microfluidic chambers by dilution, sorting, and/or segmentation. The isolated cells or viruses may be lysed directly in the microfluidic chamber, and the resulting nucleic acid purified by exposure to affinity beads. Subsequent elution of the purified nucleic acid may be followed by ligation and cell transformation, all within the same microfluidic chip. In one specific application, cell isolation, lysis, and nucleic acid purification may be performed utilizing a highly parallelized microfluidic architecture to construct gDNA and cDNA libraries.

US Patent:

20110296543, Dec 1, 2011

Filed:

Nov 26, 2008

Appl. No.:

12/324436

Inventors:

Hwai Chang - San Marcos CA, US
Eric J. Mathur - San Diego CA, US
Michelle Cayouette - San Diego CA, US
Dan E. Robertson - Belmont MA, US
Philip Hugenholtz - Berkeley CA, US
Falk Warnecke - Berkeley CA, US
Jared R. Leadbetter - Altadena CA, US
Natalia Ivanova - Clayton CA, US
Peter Luginbuhl - San Diego CA, US
Don Hutchison - Encinitas CA, US

Assignee:

The University of California - Oakland CA
California Institute Technology - Pasadena CA
Verenium Corporation - San Diego CA

International Classification:

A01K 67/00

US Classification:

800 13

Abstract:

The invention provides polypeptides, including enzymes, structural proteins and binding proteins, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. Polypeptides, including enzymes and antibodies, and nucleic acids of the invention can be used in industrial, experimental, food and feed processing, nutritional and pharmaceutical applications, e.g., for food and feed supplements, colorants, neutraceuticals, cosmetic and pharmaceutical needs. Polypeptides of the invention can be used in food processing, brewing, bath additives, alcohol production, peptide synthesis, enantioselectivity, hide preparation in the leather industry, waste management and animal degradation, silver recovery in the photographic industry, medical treatment, silk degumming, biofilm degradation, biomass conversion to a biofuel (e.g., a bioethanol, biomethanol, biopropanol or biobutanol, a biodiesel, etc.), biodefense, antimicrobial agents and disinfectants, personal care and cosmetics, biotech reagents, in corn wet milling and pharmaceuticals such as digestive aids and anti-inflammatory (anti-phlogistic) agents.

US Patent:

7335352, Feb 26, 2008

Filed:

Jun 3, 2004

Appl. No.:

10/861224

Inventors:

Jared Leadbetter - Altadena CA, US
Jean J. Huang - Pasadena CA, US

Assignee:

California Institute of Technology - Pasadena CA

International Classification:

A01N 63/00

US Classification:

424 934

Abstract:

Screening assays that allow for the identification of agents that increase acyl homoserine lactone (AHL) acylase expression and/or AHL acylase activity in γ-proteobacteria such as. Such agents are useful, for example, for inhibiting quorum sensing activity of such bacteria by increasing degradation of long chain, but not short chain, AHLs and, therefore, can be useful for treating infections by such bacteria.

US Patent:

20190009272, Jan 10, 2019

Filed:

Jan 13, 2017

Appl. No.:

15/406451

Inventors:

- Pasadena CA, US
Vincent Studer - Paris, FR
W. French Anderson - Chino CA, US
Stephen R. Quake - Stanford CA, US
Jared Leadbetter - Altadena CA, US

International Classification:

B01L 3/00
C12Q 1/6806

Abstract:

Nucleic acid from cells and viruses sampled from a variety of environments may purified and expressed utilizing microfluidic techniques. In accordance with one embodiment of the present invention, individual or small groups of cells or viruses may be isolated in microfluidic chambers by dilution, sorting, and/or segmentation. The isolated cells or viruses may be lysed directly in the microfluidic chamber, and the resulting nucleic acid purified by exposure to affinity beads. Subsequent elution of the purified nucleic acid may be followed by ligation and cell transformation, all within the same microfluidic chip. In one specific application, cell isolation, lysis, and nucleic acid purification may be performed utilizing a highly parallelized microfluidic architecture to construct gDNA and cDNA libraries.

US Patent:

20150238960, Aug 27, 2015

Filed:

Sep 23, 2014

Appl. No.:

14/494284

Inventors:

- Pasadena CA, US
Vincent Studer - Paris, FR
W. French Anderson - San Marino CA, US
Stephen R. Quake - Stanford CA, US
Jared Leadbetter - Altadena CA, US

International Classification:

B01L 3/00
C12Q 1/68

Abstract:

Nucleic acid from cells and viruses sampled from a variety of environments may purified and expressed utilizing microfluidic techniques. In accordance with one embodiment of the present invention, individual or small groups of cells or viruses may be isolated in microfluidic chambers by dilution, sorting, and/or segmentation. The isolated cells or viruses may be lysed directly in the microfluidic chamber, and the resulting nucleic acid purified by exposure to affinity beads. Subsequent elution of the purified nucleic acid may be followed by ligation and cell transformation, all within the same microfluidic chip. In one specific application, cell isolation, lysis, and nucleic acid purification may be performed utilizing a highly parallelized microfluidic architecture to construct gDNA and cDNA libraries.