Frances S. Eigler - Potomac MD Jacque Georger - Springfield VA Suresh K. Bhatia - Alexandria VA Jeff Calvert - Burke VA Reinhard Bredehorst - Washington DC
International Classification:
C12N 1114 C12N 1104 G01N 33551 G01N 33549
US Classification:
435176
Abstract:
Active agents such as proteins are covalently immobilized on substrates carrying hydroxyl groups. A silane is bound to the substrate and coupled to a heterobifunctional crosslinker at one functional group leaving a free functional group, different than the first group, to which a protein is bound while retaining high protein functionality. Preferably, the silane has a functional group which reacts with the hydroxyl group of the substrate and a thiol terminal group which reacts with a functional group of a heterobifunctional crosslinking agent which contains a succinimide group that reacts with an amino group of the active agent. Bound active agents such as proteins are useful as biosensors or reactants in a variety of applications including bioassays.
Surface Modification To Create Regions Resistant To Adsorption Of Biomolecules
Frances S. Ligler - Potomac MD Suresh Bhatia - Burke VA Jacque Georger - Springfield VA Jeff Calvert - Burke VA Charles Dulcey - Washington DC
Assignee:
The United States of America as represented by the Secretary of the Navy - Washington DC Geo-Centers, Inc. - Newton Centre MA
International Classification:
G03C 173 C12N 1106
US Classification:
430272
Abstract:
Irradiating, with ultraviolet light, surfaces which contain thiol groups, epoxy groups, or vicinal diol groups, results in surfaces which exhibit a reduced adsorption of biomolecules. In the case of surfaces having thiol groups such irradiation also results in a reduced capacity for the bonding of heterobifunctional crosslinking reagents. Such irradiation may be carried out in a patternwise fashion to obtain patterned surfaces.
Method For Surface Modification To Create Regions Resistant To Adsorption Of Biomolecules
Frances S. Ligler - Potomac MD Suresh Bhatia - Burke VA Jacque Georger - Springfield VA Jeff Calvert - Burke VA Charles Dulcey - Washington DC
Assignee:
The United States of America as represented by the Secretary of the Navy - Washington DC GEO-Centers, Inc. - Newton Centre MA
International Classification:
G03C 300
US Classification:
430 17
Abstract:
Irradiating, with ultraviolet light, surfaces which contain thiol groups, epoxy groups, or vicinal diol groups, results in surfaces which exhibit a reduced adsorption of biomolecules. In the case of surfaces having thiol groups such irradiation also results in a reduced capacity for the bonding of heterobifunctional crosslinking reagents. Such irradiation may be carried out in a patternwise fashion to obtain patterned surfaces.
Name / Title
Company / Classification
Phones & Addresses
Jeff Calvert Principal
Libertarian Party of Texas Nonclassifiable Establishments