Jeffrey D Mitchell

US Patent:

20090124023, May 14, 2009

Filed:

May 11, 2007

Appl. No.:

12/227321

Inventors:

Ailan Guo - Burlington MA, US
Jeffrey Mitchell - Nashua NH, US
Peter Hornbeck - Magnolia MA, US

International Classification:

G01N 33/566
C07K 16/18

US Classification:

436501, 5303879

Abstract:

The invention discloses 432 novel acetylation sites identified in signal transduction proteins and pathways underlying human protein acetylation signaling pathways, and provides acetylation-site specific antibodies and heavy-isotope labeled peptides (AQUA peptides) for the selective detection and quantification of these acetylated sites/proteins, as well as methods of using the reagents for such purpose. Among the acetylation sites identified are sites occurring in the following protein types: Acetyltransferases, Adaptor/Scaffold proteins, Actin binding proteins, Adhesion proteins, Apoptosis proteins, Calcium-binding proteins, Cell Cycle Regulation proteins, Cell Surface proteins, DNA binding proteins, DNA replication proteins, Channel proteins, Chaperone proteins, Cellular Metabolism enzymes, Cytoskeletal proteins, DNA repair proteins, Endoplasmic reticulum proteins, Enzyme proteins, G protein and GTPase Activating proteins, Guanine Nucleotide Exchange Factors, Helicase proteins, Isomerase proteins, Extracelluar matrix proteins, Hydrolases, Ligase proteins, Lipid kinases, Inhibtor proteins, Lipid Binding proteins and Lyases.

US Patent:

20090325312, Dec 31, 2009

Filed:

May 11, 2007

Appl. No.:

12/227320

Inventors:

Ailan Guo - Burlington MA, US
Peter Hornbeck - Magnolia MA, US
Jeffrey Mitchell - Nashua NH, US
Yu Li - Andover MA, US

International Classification:

G01N 33/566
C07K 16/18

US Classification:

436501, 5303879

Abstract:

The invention discloses novel acetylation sites identified in signal transduction proteins and pathways underlying human protein acetylation signaling pathways, and provides acetylation-site specific antibodies and heavy-isotope labeled peptides (AQUA peptides) for the selective detection and quantification of these acetylated sites/proteins, as well as methods of using the reagents for such purpose. Among the acetylation sites identified are sites occurring in the following protein types: Methyltransferases, Transcription factors, Transcription coactivators, Translation initiation complex proteins, Oxireductases, Protein kinases, RNA binding proteins, Secreted proteins, Transferases, Transporter proteins, Ubiquitin conjugating system proteins, Motor proteins, Phosphotases, Proteases, Phospholipases, Receptor proteins and Vesicle proteins.

US Patent:

46480307, Mar 3, 1987

Filed:

Sep 22, 1983

Appl. No.:

6/534782

Inventors:

Frank C. Bomba - Andover MA
Dileep P. Bhandarkar - Westford MA
J. J. Grady - Woburn MA
Stanley A. Lackey - Littleton MA
Jeffrey W. Mitchell - Maynard MA
Reinhard Schumann - Stow MA

Assignee:

Digital Equipment Corporation - Maynard MA

International Classification:

G06F 1300
G06F 1314

US Classification:

364200

Abstract:

One of a plurality of devices on a common communications path (68) has a local memory (54) that is accessible by other devices on the common communications path (68). Another device on the common communications path (68) may include a cache memory (190) that keeps copies of certain of the data contained by the local memory (54). If another device on the common communications path (68) accesses the local memory (54), the cache (190) is kept apprised of this fact by monitoring of the common communications path (68), and it sets an internal flag to indicate that the data involved may not be valid. However, the contents of memory 54 may also be accessed by means of a processor (50) without using the common communications path (68). Accordingly, provisions are made to send an invalidate signal over the common communications path (68) when a non-path access of the local memory (54) has been made to a location to which access was previously afforded over the common communications path ( 68). In this way, non-path accesses of a local memory can be permitted, yet proper invalidation of cache memories can be performed in a simple manner.

US Patent:

20210017553, Jan 21, 2021

Filed:

Jun 30, 2020

Appl. No.:

16/917099

Inventors:

- NEW YORK NY, US
Jeffrey Joseph MITCHELL - NASHUA NH, US
Bhanu Chandra MULUKUTLA - LAWRENCE MA, US
Pamela Mary PEGMAN - WESTON MA, US

Assignee:

PFIZER INC. - NEW YORK NY

International Classification:

C12P 7/62
C12P 7/42
C12P 7/52
C07K 16/00
C12P 7/56
C12P 7/40
C12P 17/18

Abstract:

The invention relates to a method of cell culture where the cells are modified to reduce the level of synthesis of growth and/or productivity inhibitors by the cell. The invention also relates to a method of cell culture for improving cell growth and productivity, in particular in fed-batch culture of mammalian cells at high cell density. The invention further relates to a method of producing cells with improved cell growth and/or productivity in cell culture and to cells obtained or obtainable by such methods.

US Patent:

20200056190, Feb 20, 2020

Filed:

Mar 9, 2018

Appl. No.:

16/492793

Inventors:

- NEW YORK NY, US
Jeffrey Joseph MITCHELL - Nashua NH, US
Bhanu Chandra MULUKUTLA - Lawrence MA, US
Pamela Mary PEGMAN - Weston MA, US

Assignee:

PFIZER INC. - NEW YORK NY

International Classification:

C12N 15/62
C12N 9/02
C12N 9/88

Abstract:

Provided herein is a tyrosine selection marker system, and uses thereof. In some embodiments, nucleic acid constructs, vectors, host cells and related compositions and methods for generating and selecting tyrosine prototroph cells are provided.

US Patent:

20180265904, Sep 20, 2018

Filed:

Sep 22, 2016

Appl. No.:

15/762345

Inventors:

- NEW YORK NY, US
JEFFREY JOSEPH MITCHELL - NASHUA NH, US
BHANU CHANDRA MULUKUTLA - LAWRENCE MA, US
PAMELA MARY PEGMAN - WESTON MA, US

Assignee:

PFIZER INC. - NEW YORK NY

International Classification:

C12P 7/62
C12P 7/42
C12P 7/52

Abstract:

The invention relates to a method of cell culture where the cells are modified to reduce the level of synthesis of growth and/or productivity inhibitors by the cell. The invention also relates to a method of cell culture for improving cell growth and productivity, in particular in fed-batch culture of mammalian cells at high cell density. The invention further relates to a method of producing cells with improved cell growth and/or productivity in cell culture and to cells obtained or obtainable by such methods.