Jeffrey T Mohr

US Patent:

7732152, Jun 8, 2010

Filed:

Nov 30, 2006

Appl. No.:

11/565363

Inventors:

Nicholas L. Abbott - Madison WI, US
Jeffrey C. Mohr - Madison WI, US
Christopher J. Murphy - Madison WI, US
Elise Huang - Fitchburg WI, US
Juan J. De Pablo - Madison WI, US
Sean P. Palecek - Madison WI, US
Nathan A. Lockwood - Minneapolis MN, US

Assignee:

Wisconsin Alumni Research Foundation - Madison WI

International Classification:

G01N 33/53
G01N 33/567
C12N 5/00
C12N 5/02
G01N 33/566

US Classification:

435 721, 435 72, 435325, 435394, 436501

Abstract:

The present invention is directed to liquid crystalline substrates useful in the culture of cells and methods of their use. In certain embodiments, the invention provides methods and devices for imaging changes (e. g. , reorganization) of extracellular matrix components by living cells.

US Patent:

7947500, May 24, 2011

Filed:

May 11, 2010

Appl. No.:

12/777320

Inventors:

Nicholas L. Abbott - Madison WI, US
Jeffrey C. Mohr - Madison WI, US
Christopher J. Murphy - Madison WI, US
Elise Huang - Fitchburg WI, US
Juan J. DePablo - Madison WI, US
Nathan A. Lockwood - Minneapolis MN, US
Sean P. Palecek - Madison WI, US

Assignee:

Wisconsin Alumni Research Foundation - Madison WI

International Classification:

G01N 33/53
G01N 33/567
G01N 33/566
C12N 5/00
C12N 5/02

US Classification:

435395, 435 72, 435325, 435408, 435 721, 436501

Abstract:

The present invention is directed to liquid crystalline substrates useful in the culture of cells and methods of their use. In certain embodiments, the invention provides methods and devices for imaging changes (e. g. , reorganization) of extracellular matrix components by living cells.

US Patent:

8097457, Jan 17, 2012

Filed:

Apr 13, 2011

Appl. No.:

13/085846

Inventors:

Nicholas L. Abbott - Madison WI, US
Jeffrey C. Mohr - Madison WI, US
Christopher J. Murphy - Madison WI, US
Elise Huang - Fitchburg WI, US
Juan J. DePablo - Madison WI, US
Nathan A. Lockwood - Minneapolis MN, US
Sean P. Palecek - Madison WI, US

Assignee:

Wisconsin Alumni Research Foundation - Madison WI

International Classification:

G01N 33/53
G01N 33/567
G01N 33/566
C12N 5/00
C12N 5/02

US Classification:

435395, 435 72, 435325, 435408, 435 721, 436501

Abstract:

The present invention is directed to liquid crystalline substrates useful in the culture of cells and methods of their use. In certain embodiments, the invention provides methods and devices for imaging changes (e. g. , reorganization) of extracellular matrix components by living cells.

US Patent:

20080026460, Jan 31, 2008

Filed:

Jun 20, 2007

Appl. No.:

11/765831

Inventors:

Sean Palecek - Verona WI, US
Jeffrey Mohr - Madison WI, US
Juan De Pablo - Madison WI, US

International Classification:

C12N 5/02
C12M 1/16
C12N 5/08

US Classification:

435366000, 435287900, 435374000, 435395000

Abstract:

A three-dimensional microwell system that supports long term embryonic stem cell (ESCs) culture and formation of homogeneous embryoid bodies (EBs) is described. Microwell-cultured ESCs remain viable and undifferentiated for several weeks in culture and maintain undifferentiated replication when passaged to Matrigel-coated, tissue culture-treated polystyrene dishes. Microwell-cultured ESCs maintain pluripotency, differentiating to each of the three embryonic germ layers. ESC aggregates released from microwells can be passaged for undifferentiated replication or differentiated to monodisperse EBs. The ability to constrain ESC growth in three dimensions advantageously provides for more efficient, reproducible culture of undifferentiated cells, high-throughput screening, and the ability to direct ESC differentiation by generating monodisperse EBs of a desired size and shape.

US Patent:

20080220520, Sep 11, 2008

Filed:

Feb 18, 2008

Appl. No.:

12/032986

Inventors:

Sean P. Palecek - Madison WI, US
Juan J. DePablo - Madison WI, US
Jeffrey C. Mohr - Madison WI, US
Lin Ji - Madison WI, US

International Classification:

C12N 5/06
C12M 1/00

US Classification:

435374, 4353071

Abstract:

The present invention relates to methods and structures for preparing stem cells for use in cryopreservation methods. Stem cell colonies are provided between first and second matrix portions and are exposed to a carbohydrate-containing cryoprotecting medium and a freezing medium. The methods of the invention yield cryopreserved cells that maintain cell viability and exhibit limited cell differentiation after freezing and thawing, facilitating storage, shipping and handling of embryonic stem cell stocks and lines for research and therapeutics.

US Patent:

20100197013, Aug 5, 2010

Filed:

Nov 9, 2009

Appl. No.:

12/615044

Inventors:

Timothy J. Kamp - Madison WI, US
Jianhua Zhang - Madison WI, US
Jeffrey C. Mohr - Glenview IL, US
Juan J. Depablo - Madison WI, US
Sean P. Palecek - Verona WI, US

International Classification:

C12N 5/071
C12N 5/02
C12N 5/00
C40B 40/14

US Classification:

435366, 435377, 435325, 506 20, 435374

Abstract:

A three-dimensional microwell system that supports long term pluripotent cell culture and formation of homogeneous embryoid bodies (EBs) is described. Microwell-cultured pluripotent cells remain viable and undifferentiated for several weeks in culture and maintain undifferentiated replication when passaged to Matrigel-coated, tissue culture-treated polystyrene dishes. Microwell-cultured pluripotent cells maintain pluripotency, differentiating to each of the three embryonic germ layers. Pluripotent cell aggregates released from microwells can be passaged for undifferentiated replication or differentiated to monodisperse EBs. The ability to constrain pluripotent cell growth in three dimensions advantageously provides for more efficient, reproducible culture of undifferentiated cells, high-throughput screening, and the ability to direct pluripotent cell differentiation by generating monodisperse EBs of a desired size and shape. Cardiomyocyte-rich EBs are obtained from pluripotent cells cultured in microwells of defined size and shape.