Jeffrey D Stuart

US Patent:

7790442, Sep 7, 2010

Filed:

Jul 19, 2007

Appl. No.:

11/779963

Inventors:

Jeffrey A. Stuart - Columbia CT, US
Duane Marcy - Syracuse NY, US
Robert R. Birge - Coventry CT, US

Assignee:

Syracuse University - Syracuse NY

International Classification:

C12M 3/00

US Classification:

4352887, 422 8205, 422 8208, 435808, 436164, 436172, 436805

Abstract:

A bacteriorhodopsin based chemical sensing architecture based upon the collective response of bacteriorhodopsin and a number of its mutants; the wild type protein and a selection of genetically-engineered variants was able to respond differentially to a selection of amines. The observable response to the presence of a target chemical was manifested through a modulation of bacteriorhodopsin's photokinetic properties, which are monitored through pump-probe techniques using a custom prototype flash photolysis system. Differential responsivity exists at two levels; (1) bacteriorhodopsin proteins (wild-type and genetically-engineered variants) respond differentially upon exposure of a target chemical, and (2) the response pattern exhibited by the proteins differs from chemical to chemical. This dichotomy forms the basis for a BR-mediated chemical sensing technology that is highly sensitive and selective and may therefore discriminate between different chemicals.

US Patent:

8163539, Apr 24, 2012

Filed:

Jul 20, 2010

Appl. No.:

12/839736

Inventors:

Jeffrey A. Stuart - Columbia CT, US
Duane L. Marcy - Syracuse NY, US
Robert R. Birge - Coventry CT, US

Assignee:

Syracuse University - Syracuse NY

International Classification:

C12M 3/00

US Classification:

4352887, 422 8205, 422 8208, 435808, 436164, 436172, 436805

Abstract:

A bacteriorhodopsin based chemical sensing architecture based upon the collective response of bacteriorhodopsin and a number of its mutants; the wild type protein and a selection of genetically-engineered variants was able to respond differentially to a selection of amines. The observable response to the presence of a target chemical was manifested through a modulation of bacteriorhodopsin's photokinetic properties, which are monitored through pump-probe techniques using a custom prototype flash photolysis system. Differential responsivity exists at two levels; (1) bacteriorhodopsin proteins (wild-type and genetically-engineered variants) respond differentially upon exposure of a target chemical, and (2) the response pattern exhibited by the proteins differs from chemical to chemical. This dichotomy forms the basis for a BR-mediated chemical sensing technology that is highly sensitive and selective and may therefore discriminate between different chemicals.

US Patent:

20080035897, Feb 14, 2008

Filed:

Jul 16, 2007

Appl. No.:

11/778377

Inventors:

Jeffrey Stuart - Columbia CT, US

Assignee:

SYRACUSE UNIVERSITY - Syracuse NY

International Classification:

G02B 5/00
C07G 17/00
C08H 1/00

US Classification:

252586000, 204157150

Abstract:

A binary optical compound formed from a preparation of proteorhodopsin, is provided. The preparation is prepared by dissolving proteorhodopsin in distilled water, where the pH is adjusted to be above 11 either by using sodium hydroxide or an appropriate buffer. The preparation is then illuminated with either white light sources, lasers, or irradiation provided from light-emitting diodes. The necessary duration is intensity dependent, varying from a few seconds to a few minutes, depending upon the source and actinic wavelength. The resulting compound has a long lifespan and may be efficiently transitioned between states.

US Patent:

20090311799, Dec 17, 2009

Filed:

Jun 15, 2009

Appl. No.:

12/484509

Inventors:

Gregory A. Sotzing - Storrs CT, US
Jeffrey A. Stuart - Columbia CT, US
Yogesh J. Ner - Willimantic CT, US

International Classification:

G01N 21/00
C07H 21/00
B29C 47/00
B29C 39/00
G21G 5/00

US Classification:

436172, 536 221, 264465, 264299, 2504921

Abstract:

Nucleic acid materials for FRET-based luminescence and methods of making and using the nucleic acid materials are provided. The nucleic acid materials provide an innovative and synergistic combination of three disparate elements: a nucleic acid material, the processing technique for forming a nucleic acid material into films, fibers, nanofibers, or non-woven meshes, and nonradiative energy transfer. This combination can be formed into electrospun fibers, nanofibers, and non-woven meshes of a nucleic acid material-cationic lipid complex with encapsulated chromophores capable of nonradiative energy transfer such as efficient Förster Resonance Energy Transfer (FRET).