Jeffrey C Thornton

US Patent:

20030119070, Jun 26, 2003

Filed:

Jul 29, 2002

Appl. No.:

10/209323

Inventors:

Andrew Schaeffer - La Mirada CA, US
Janet Tseng-Law - Aliso Viejo CA, US
Jeffrey Thornton - Rancho Santa Margarita CA, US
Dennis Van Epps - Coto de Caza CA, US

Assignee:

Nexell Therapeutics, Inc.

International Classification:

G01N033/53
G01N033/567
G01N033/574
C12N005/08
C07K016/30

US Classification:

435/007200, 530/388220, 530/391100, 435/372000, 435/007230

Abstract:

The present invention provides peptide-ligand conjugates containing a peptide, where a free form of the peptide is capable of displacing a second ligand specific for the peptide. The invention also provides a method of selecting a cell. The method includes the steps of contacting a cell population with a peptide-ligand conjugate, wherein the conjugate comprises a first ligand specific for a cell in the population conjugated to a peptide capable of displacing a second ligand specific for the peptide, thereby forming a cell-conjugate complex; contacting the peptide-ligand conjugate with the second ligand; and isolating the cell-conjugate complex. The method can further include the step of adding a free form of the peptide to release the cell from the complex. The invention also provides a subpopulation of cells containing two or more specifically isolated cells and methods of isolating cell subpopulations. The invention additionally provides a method of diagnosing a condition using peptide-ligand conjugates. The invention further provides a kit for isolating a cell containing a peptide capable of displacing an antibody specific for the peptide and the antibody.

US Patent:

20030219445, Nov 27, 2003

Filed:

Sep 25, 2002

Appl. No.:

10/256277

Inventors:

Andrew Schaeffer - La Mirada CA, US
Janet Tseng-Law - Aliso Viejo CA, US
Jeffrey Thornton - Rancho Santa Margarita CA, US

Assignee:

Nexell Therapeutics, Inc.

International Classification:

A61K039/395
C07K016/46

US Classification:

424/178100, 530/391100

Abstract:

The present invention provides peptide-ligand conjugates containing a peptide, where a free form of the peptide is capable of displacing a second ligand specific for the peptide. The invention also provides a method of selecting a cell. The method includes the steps of contacting a cell population with a peptide-ligand conjugate, wherein the conjugate comprises a first ligand specific for a cell in the population conjugated to a peptide capable of displacing a second ligand specific for the peptide, thereby forming a cell-conjugate complex; contacting the peptide-ligand conjugate with the second ligand; and isolating the cell-conjugate complex. The method can further include the step of adding a free form of the peptide to release the cell from the complex. The invention additionally provides a method of diagnosing a condition using peptide-ligand conjugates. The invention further provides a kit for isolating a cell containing a peptide capable of displacing an antibody specific for the peptide and the antibody.