Jennifer M O'Neil

US Patent:

20130203607, Aug 8, 2013

Filed:

Mar 14, 2013

Appl. No.:

13/828049

Inventors:

Life Technologies Corporation - Carlsbad CA, US
Kai Qin LAO - Pleasanton CA, US
Jennifer O'NEIL - Peabody MA, US
Jennifer KUNKEL - Amesbury MA, US
Kellie HALEY - Peabody MA, US
Rachel KASINSKAS - Amesbury MA, US
Zhaochun MA - Sunnyvale CA, US
Pius BRZOSKA - Woodside CA, US

Assignee:

LIFE TECHNOLOGIES CORPORATION - Carlsbad CA

International Classification:

C12Q 1/68

US Classification:

506 2, 435 612

Abstract:

In some embodiments, the present teachings provide methods for paired end sequencing. In some embodiment, a polynucleotide template to be subjected to paired end sequencing comprises at least one cross linking moiety and at least one scissile moiety. In some embodiments, a paired end sequencing reaction comprises (a) a forward sequencing step, (b) a cleavage step, and (c) a reverse sequencing step. In some embodiments, a paired end sequencing reaction comprises (a) a forward sequencing step, (b) a cross-linking step, (c) a cleavage step, and (d) a reverse sequencing step.

US Patent:

20130225421, Aug 29, 2013

Filed:

Mar 15, 2013

Appl. No.:

13/842296

Inventors:

LIFE TECHNOLOGIES CORPORATION - Carlsbad CA, US
David RUFF - San Francisco CA, US
Shiaw-Min CHEN - Fremont CA, US
Jennifer O'NEIL - Peabody MA, US
Rachel KASINSKAS - Amesbury MA, US
Jonathan ROTHBERG - Guilford CT, US

Assignee:

LIFE TECHNOLOGIES CORPORATION - Carlsbad CA

International Classification:

C12Q 1/68

US Classification:

506 2, 506 26

Abstract:

In some embodiments, the present teachings provide methods for nucleic acid amplification, comprising forming a reaction mixture, and subjecting the reaction mixture to conditions suitable for nucleic acid amplification. In some embodiments, methods for nucleic acid amplification include subjecting the nucleic acid to be amplified to partially denaturing conditions. In some embodiments, methods for nucleic acid amplification include amplifying without fully denaturing the nucleic acid that is amplified. In some embodiments, the methods for nucleic acid amplification employ an enzyme that catalyzes homologous recombination and a polymerase. In some embodiments, methods for nucleic acid amplification can be conducted in a single reaction vessel. In some embodiments, methods for nucleic acid amplification can be conducted in a single continuous liquid phase of a reaction mixture, without need for compartmentalization of the reaction mixture or immobilization of reaction components. In some embodiments, methods for nucleic acid amplification comprise a amplifying at least one polynucleotide onto a surface under isothermal amplification conditions, optionally in the presence of a polymer. The polymer can include a sieving agent and/or a diffusion-reducing agent.