Jennifer P O'Neil

US Patent:

20120156728, Jun 21, 2012

Filed:

Dec 16, 2011

Appl. No.:

13/328844

Inventors:

Bin Li - Palo Alto CA, US
Kai Lao - Pleasanton CA, US
Jennifer O'Neil - Peabody MA, US
Jennifer Kunkel - Amesbury MA, US
Kellie Haley - Peabody MA, US
Rachel Kasinskas - Amesbury MA, US
Zhaochun Ma - Sunnyvale CA, US
Pius Brzoska - Woodside CA, US

Assignee:

LIFE TECHNOLOGIES CORPORATION - Carlsbad CA

International Classification:

C12P 19/34

US Classification:

435 911

Abstract:

Novel methods of generating a localized population of immobilized clonal amplicons on a support are provided.

US Patent:

20130281307, Oct 24, 2013

Filed:

Jun 20, 2013

Appl. No.:

13/923232

Inventors:

David RUFF - San Francisco CA, US
Jennifer O'NEIL - Peabody MA, US
Rachel KASINSKAS - Amesbury MA, US
Shiaw-Min CHEN - Fremont CA, US
Jonathan ROTHBERG - Guilford CT, US

International Classification:

C12Q 1/68

US Classification:

506 2, 506 26

Abstract:

In some embodiments, the present teachings provide methods for nucleic acid amplification, comprising forming a reaction mixture, and subjecting the reaction mixture to conditions suitable for nucleic acid amplification. In some embodiments, methods for nucleic acid amplification include subjecting the nucleic acid to be amplified to partially denaturing conditions. In some embodiments, methods for nucleic acid amplification include amplifying without fully denaturing the nucleic acid that is amplified. In some embodiments, the methods for nucleic acid amplification employ an enzyme that catalyzes homologous recombination and a polymerase. In some embodiments, methods for nucleic acid amplification can be conducted in a single reaction vessel. In some embodiments, methods for nucleic acid amplification can be conducted in a single continuous liquid phase of a reaction mixture, without need for compartmentalization of the reaction mixture or immobilization of reaction components. In some embodiments, methods for nucleic acid amplification comprise a amplifying at least one polynucleotide onto a surface under isothermal amplification conditions, optionally in the presence of a polymer. The polymer can include a sieving agent and/or a diffusion-reducing agent.

US Patent:

20140080717, Mar 20, 2014

Filed:

Sep 10, 2013

Appl. No.:

14/023361

Inventors:

David RUFF - San Francisco CA, US
Shiaw-Min CHEN - Fremont CA, US
Jennifer O'NEIL - Peabody MA, US
Rachel KASINSKAS - Amesbury MA, US
Jonathan ROTHBERG - Guilford CT, US
Wolfgang HINZ - Killingworth CT, US

Assignee:

Life Technologies Corporation - Carlsbad CA

International Classification:

C12Q 1/68

US Classification:

506 2, 506 26

Abstract:

In some embodiments, the present teachings provide methods for nucleic acid amplification, comprising forming a reaction mixture, and subjecting the reaction mixture to conditions suitable for nucleic acid amplification. In some embodiments, methods for nucleic acid amplification include subjecting the nucleic acid to be amplified to partially denaturing conditions. In some embodiments, methods for nucleic acid amplification include amplifying without fully denaturing the nucleic acid that is amplified. In some embodiments, the methods for nucleic acid amplification employ an enzyme that catalyzes homologous recombination and a polymerase. In some embodiments, methods for nucleic acid amplification can be conducted in a single reaction vessel. In some embodiments, methods for nucleic acid amplification can be conducted in a single continuous liquid phase of a reaction mixture, without need for compartmentalization of the reaction mixture or immobilization of reaction components. In some embodiments, methods for nucleic acid amplification comprise a amplifying at least one polynucleotide onto a surface under isothermal amplification conditions, optionally in the presence of a polymer. The polymer can include a sieving agent and/or a diffusion-reducing agent.

US Patent:

20210261929, Aug 26, 2021

Filed:

Apr 27, 2021

Appl. No.:

17/302192

Inventors:

- Carlsbad CA, US
David RUFF - Oxford, GB
Shiaw-Min CHEN - San Jose CA, US
Jennifer O'NEIL - Wakefield MA, US
Rachel KASINSKAS - Amesbury MA, US
Jonathan ROTHBERG - Guilford CT, US
Bin LI - Palo Alto CA, US
Kai Qin LAO - Pleasanton CA, US

International Classification:

C12N 9/12
C12Q 1/6853
C12Q 1/6844
C12Q 1/6855
C12Q 1/686

Abstract:

The present disclosure provides methods, compositions, kits and systems for nucleic acid amplification. In some embodiments, nucleic acid amplification methods include subjecting the nucleic acid to be amplified to partially denaturing conditions. In some embodiments, nucleic acid amplification methods include amplifying without fully denaturing the nucleic acid that is amplified. In some embodiments, the nucleic acid amplification method employs an enzyme that catalyzes homologous recombination and a polymerase. In some embodiments, methods for nucleic acid amplification can be conducted in a single reaction vessel and/or in a single continuous liquid phase of a reaction mixture, without need for compartmentalization of the reaction mixture or immobilization of reaction components. In some embodiments, methods for nucleic acid amplification comprise amplifying at least one polynucleotide onto a surface under isothermal amplification conditions, optionally in the presence of a polymer which can include a sieving agent and/or a diffusion-reducing agent.

US Patent:

20210032691, Feb 4, 2021

Filed:

Oct 13, 2020

Appl. No.:

16/949083

Inventors:

- Carlsbad CA, US
Kai Qin LAO - Pleasanton CA, US
Jennifer O'NEIL - Wakefield MA, US
Jennifer KUNKEL - Burlington MA, US
Kellie HALEY - Peabody MA, US
Rachel KASINSKAS - Amesbury MA, US
Zhaochun MA - Sunnyvale CA, US
Pius BRZOSKA - Woodside CA, US

International Classification:

C12Q 1/6853
C12Q 1/6844
C12Q 1/6806
C12P 19/34
C12Q 1/6869
C12Q 1/6874
C12Q 1/6834
C12Q 1/46

Abstract:

Novel methods of generating a localized population of immobilized clonal amplicons on a support are provided.

US Patent:

20190338258, Nov 7, 2019

Filed:

Jun 14, 2019

Appl. No.:

16/442341

Inventors:

- Carlsbad CA, US
David RUFF - San Francisco CA, US
Shiaw-Min CHEN - San Jose CA, US
Jennifer O'NEIL - Wakefield MA, US
Rachel KASINSKAS - Amesbury MA, US
Jonathan ROTHBERG - Guilford CT, US
Bin LI - Palo Alto CA, US
Kai Qin LAO - Pleasanton CA, US

International Classification:

C12N 9/12
C12Q 1/6844
C12Q 1/6853
C12Q 1/686
C12Q 1/6855

Abstract:

The present disclosure provides methods, compositions, kits and systems for nucleic acid amplification. In some embodiments, nucleic acid amplification methods include subjecting the nucleic acid to be amplified to partially denaturing conditions. In some embodiments, nucleic acid amplification methods include amplifying without fully denaturing the nucleic acid that is amplified. In some embodiments, the nucleic acid amplification method employs an enzyme that catalyzes homologous recombination and a polymerase. In some embodiments, methods for nucleic acid amplification can be conducted in a single reaction vessel and/or in a single continuous liquid phase of a reaction mixture, without need for compartmentalization of the reaction mixture or immobilization of reaction components. In some embodiments, methods for nucleic acid amplification comprise amplifying at least one polynucleotide onto a surface under isothermal amplification conditions, optionally in the presence of a polymer which can include a sieving agent and/or a diffusion-reducing agent.

US Patent:

20190264272, Aug 29, 2019

Filed:

Mar 12, 2019

Appl. No.:

16/351194

Inventors:

- Carlsbad CA, US
Kai Lao - Pleasanton CA, US
Jennifer O'NEIL - Wakefield MA, US
Jennifer KUNKEL - Burlington MA, US
Kellie HALEY - Peabody MA, US
Rachel KASINSKAS - Amesbury MA, US
Zhaochun MA - Sunnyvale CA, US
Pius BRZOSKA - Woodside CA, US

International Classification:

C12Q 1/6853
C12Q 1/6834
C12Q 1/6874
C12Q 1/46
C12Q 1/6844
C12Q 1/6869
C12P 19/34

Abstract:

Novel methods of generating a localized population of immobilized clonal amplicons on a support are provided.

US Patent:

20170067098, Mar 9, 2017

Filed:

Sep 26, 2016

Appl. No.:

15/276713

Inventors:

- Carlsbad CA, US
Kai Qin LAO - Pleasanton CA, US
Jennifer O'NEIL - Wakefield MA, US
Jennifer KUNKEL - Burlington CA, US
Kellie HALEY - Peabody MA, US
Rachel KASINSKAS - Amesbury CA, US
Zhaochun MA - Sunnyvale CA, US
Pius BRZOSKA - Woodside CA, US

International Classification:

C12Q 1/68
C12P 19/34

Abstract:

Novel methods of generating a localized population of immobilized clonal amplicons on a support are provided.