Jeremy K Stuart

US Patent:

20090099027, Apr 16, 2009

Filed:

Aug 22, 2008

Appl. No.:

12/197132

Inventors:

Douglas P. GREINER - Fremont CA, US
Jeremy STUART - Charlotte NC, US
Meng C. TAING - Hayward CA, US
Steven M. MENCHEN - Fremont CA, US

Assignee:

APPLIED BIOSYSTEMS INC. - Foster City CA

International Classification:

C40B 20/04
C40B 50/18
C40B 40/04

US Classification:

506 4, 506 32, 506 15

Abstract:

Methods of modifying a nucleophilic surface of a support are described. The methods involve reacting a multifunctional electrophilic reagent with nucleophilic groups on the surface of the support. The resulting electrophilic surface can be used for the covalent attachment of particles (e.g. beads) having nucleophilic functional groups. For example, nucleic acid templates with nucleophilic (e.g., amine) groups can be attached to a surface of the particles. The nucleophilic groups on the nucleic acid templates can then be used to attach the particles to the modified surface of the support. The resulting support-bound particles can be used to analyze (e.g., sequence) the nucleic acid templates on the particles.

Address:

704 228 Ave NE #112, Sammamish, WA 98074

Phone:

(925)8046624

VIN:

5TDDK3EH1BS045217

Make:

TOYOTA

Model:

HIGHLANDER

Year:

2011