Jessica V Parks

US Patent:

20210108224, Apr 15, 2021

Filed:

Dec 22, 2020

Appl. No.:

17/131057

Inventors:

- Durham NC, US
Rebecca E. Thayer - Morrisville NC, US
Jessica Parks - Raleigh NC, US

Assignee:

AgBiome, Inc. - Durham NC

International Classification:

C12N 15/82
C07K 14/325
C07K 14/32
A01N 63/20

Abstract:

Compositions having pesticidal activity and methods for their use are provided. Compositions include isolated and recombinant polypeptide sequences having pesticidal activity, recombinant and synthetic nucleic acid molecules encoding the pesticidal polypeptides, DNA constructs comprising the nucleic acid molecules, vectors comprising the nucleic acid molecules, host cells comprising the vectors, and antibodies to the pesticidal polypeptides. Nucleotide sequences encoding the polypeptides provided herein can be used in DNA constructs or expression cassettes for transformation and expression in organisms of interest. The compositions and methods provided herein are useful for the production of organisms with enhanced pest resistance or tolerance. Transgenic plants and seeds comprising a nucleotide sequence that encodes a pesticidal protein of the invention are also provided. Methods are provided for producing the polypeptides disclosed herein, and for using those polypeptides for controlling a pest. Methods and kits for detecting polypeptides of the invention in a sample are also included.

US Patent:

20190194266, Jun 27, 2019

Filed:

Dec 20, 2018

Appl. No.:

16/227544

Inventors:

- Durham NC, US
Rebecca E. Thayer - Morrisville NC, US
Jessica Parks - Raleigh NC, US

Assignee:

AgBiome, Inc. - Durham NC

International Classification:

C07K 14/325
C12N 15/82

Abstract:

Compositions having pesticidal activity and methods for their use are provided. Compositions include isolated and recombinant polypeptide sequences having pesticidal activity, recombinant and synthetic nucleic acid molecules encoding the pesticidal polypeptides, DNA constructs comprising the nucleic acid molecules, vectors comprising the nucleic acid molecules, host cells comprising the vectors, and antibodies to the pesticidal polypeptides. Nucleotide sequences encoding the polypeptides provided herein can be used in DNA constructs or expression cassettes for transformation and expression in organisms of interest. The compositions and methods provided herein are useful for the production of organisms with enhanced pest resistance or tolerance. Transgenic plants and seeds comprising a nucleotide sequence that encodes a pesticidal protein of the invention are also provided. Methods are provided for producing the polypeptides disclosed herein, and for using those polypeptides for controlling a pest. Methods and kits for detecting polypeptides of the invention in a sample are also included.

US Patent:

20180135045, May 17, 2018

Filed:

Jan 4, 2018

Appl. No.:

15/862184

Inventors:

- Research Triangle Park NC, US
Janice Jones - Apex NC, US
Jessica Parks - Raleigh NC, US
Rebecca E. Thayer - Morrisville NC, US
Daniel J. Tomso - Bahama NC, US
Scott Joseph Uknes - Apex NC, US
Sandy Volrath - Durham NC, US
Eric Russell Ward - Durham NC, US

Assignee:

AgBiome, Inc. - Research Triangle Park NC

International Classification:

C12N 15/10
C12Q 1/6827
C12Q 1/6869
C12Q 1/6806

Abstract:

Compositions and methods for isolating new variants of known gene sequences are provided. The methods find use in identifying variants, particularly homologs, in complex mixtures. Compositions comprise hybridization baits that hybridize to gene families of interest, particularly agricultural interest, in order to selectively enrich the polynucleotides of interest from complex mixtures. Bait sequences may be specific for a number of genes from distinct gene families of interest and may be designed to cover each gene of interest by at least 2-fold. Thus methods disclosed herein are drawn to an oligonucleotide hybridization gene capture approach for identification of new genes of interest from environmental samples. This approach bypasses the need for labor-intensive microbial strain isolation, permits simultaneous discovery of genes from multiple gene families of interest, and increases the potential to discover genes from low-abundance and unculturable organisms present in complex mixtures of environmental microbes.

US Patent:

20150191720, Jul 9, 2015

Filed:

Jan 8, 2015

Appl. No.:

14/592473

Inventors:

- Research Triangle Park NC, US
Janice Jones - Apex NC, US
Jessica Parks - Research Triangle Park NC, US
Rebecca E. Thayer - Morrisville NC, US
Daniel J. Tomso - Bahama NC, US
Scott Joseph Uknes - Apex NC, US
Sandy Volrath - Durham NC, US
Eric Russell Ward - Durham NC, US

Assignee:

AgBiome, Inc. - Research Triangle Park NC

International Classification:

C12N 15/10

Abstract:

Compositions and methods for isolating new variants of known gene sequences are provided. The methods find use in identifying variants, particularly homologs, in complex mixtures. Compositions comprise hybridization baits that hybridize to gene families of interest, particularly agricultural interest, in order to selectively enrich the polynucleotides of interest from complex mixtures. Bait sequences may be specific for a number of genes from distinct gene families of interest and may be designed to cover each gene of interest by at least 2-fold. Thus methods disclosed herein are drawn to an oligonucleotide hybridization gene capture approach for identification of new genes of interest from environmental samples. This approach bypasses the need for labor-intensive microbial strain isolation, permits simultaneous discovery of genes from multiple gene families of interest, and increases the potential to discover genes from low-abundance and unculturable organisms present in complex mixtures of environmental microbes.