Jian Jin

US Patent:

7079241, Jul 18, 2006

Filed:

Apr 5, 2001

Appl. No.:

09/827256

Inventors:

Stephen A. Empedocles - Mountain View CA, US
Andrew R. Watson - Belmont CA, US
Jian Jin - Berkeley CA, US

Assignee:

Invitrogen Corp. - Carlsbad CA

International Classification:

G01J 3/28
G01J 3/00
G01J 3/30
G01J 3/42
G01N 21/29

US Classification:

356326, 356300, 356311, 356317, 356319, 435 4, 436518, 436523, 436524, 422 8205

Abstract:

Devices, systems, kits, and methods for detecting and/or identifying a plurality of spectrally labeled bodies well-suited for performing multiplexed assays. By spectrally labeling the beads with materials which generate identifiable spectra, a plurality of beads may be identified within the fluid. Reading of the beads is facilitated by restraining the beads in arrays, and/or using a focused laser.

US Patent:

7737412, Jun 15, 2010

Filed:

Jul 12, 2005

Appl. No.:

11/571980

Inventors:

Jian Jin - Berkeley CA, US
Robert M. Glaeser - Berkeley CA, US

Assignee:

The Regents of the University of California - Oakland CA

International Classification:

G21K 5/04
H01J 37/24

US Classification:

250396R, 250309, 250311

Abstract:

A microfabricated electron phase shift element is used for modifying the phase characteristics of an electron beam passing though its center aperture, while not affecting the more divergent portion of an incident beam to selectively provide a ninety-degree phase shift to the unscattered beam in the back focal plan of the objective lens, in order to realize Zernike-type, in-focus phase contrast in an electron microscope. One application of the element is to increase the contrast of an electron microscope for viewing weakly scattering samples while in focus. Typical weakly scattering samples include biological samples such as macromolecules, or perhaps cells. Preliminary experimental images demonstrate that these devices do apply a ninety degree phase shift as expected. Electrostatic calculations have been used to determine that fringing fields in the region of the scattered electron beams will cause a negligible phase shift as long as the ratio of electrode length to the transverse feature-size aperture is about 5:1. Calculations are underway to determine the feasibility of aspect smaller aspect ratios of about 3:1 and about 2:1.

US Patent:

8405828, Mar 26, 2013

Filed:

Nov 17, 2009

Appl. No.:

12/620475

Inventors:

Stephen Empedocles - Mountain View CA, US
Andrew Watson - Belmont CA, US
Jian Jin - Berkeley CA, US

Assignee:

Life Technologies Corporation - Carlsbad CA

International Classification:

G01J 3/28
G01J 3/00
G01J 3/30
G01J 3/42
G01N 21/29

US Classification:

356326, 356300, 356311, 356317, 356319, 435 4, 436518, 436523, 436524, 422 8205

Abstract:

Devices, systems, kits, and methods for detecting and/or identifying a plurality of spectrally labeled bodies well-suited for performing multiplexed assays. By spectrally labeling the beads with materials which generate identifiable spectra, a plurality of beads may be identified within the fluid. Reading of the beads is facilitated by restraining the beads in arrays, and/or using a focused laser.

US Patent:

20020090650, Jul 11, 2002

Filed:

Oct 31, 2001

Appl. No.:

09/999780

Inventors:

Stephen Empedocles - Mountain View CA, US
Andrew Watson - Belmont CA, US
Jian Jin - Berkeley CA, US

Assignee:

Quantum Dot Corporation - Hayward CA

International Classification:

G01N033/53
G06F019/00
G01N033/48
G01N033/50

US Classification:

435/007100, 702/019000

Abstract:

Improved devices, systems, and methods for sensing and/or identifying signals from within a signal detection region are well-suited for identification of spectral codes. Large numbers of independently identifiable spectral codes can be generated by quite small bodies, and a plurality of such bodies or probes may be present within a detection region. Simultaneously imaging of identifiable spectra from throughout the detection region allows the probes to be identified. As the identifiable spectra can be treated as being generated from a point source within a much larger detection field, a prism, diffractive grading, holographic transmissive grading, or the like can spectrally disperse the images of the labels across a sensor surface. A CCD can identify the relative wavelengths of signals making up the spectra. Absolute signal wavelengths may be identified by determining positions of the labels, by an internal wavelength reference within the spectra, or the like.

US Patent:

20060244963, Nov 2, 2006

Filed:

Jun 22, 2006

Appl. No.:

11/425851

Inventors:

Stephen Empedocles - Mountain View CA, US
Andrew Watson - Belont CA, US
Jian Jin - Berkeley CA, US

International Classification:

G01J 3/28
G01J 3/00

US Classification:

356326000, 356300000

Abstract:

Devices, systems, kits, and methods for detecting and/or identifying a plurality of spectrally labeled bodies well-suited for performing multiplexed assays. By spectrally labeling the beads with materials which generate identifiable spectra, a plurality of beads may be identified within the fluid. Reading of the beads is facilitated by restraining the beads in arrays, and/or using a focused laser.

US Patent:

20120043208, Feb 23, 2012

Filed:

Jul 5, 2011

Appl. No.:

13/176704

Inventors:

Jian Jin - Berkeley CA, US
Mark D. Biggin - Berkeley CA, US
Robert A. Nordmeyer - San Leandro CA, US
Ming Dong - El Cerrito CA, US
Earl W. Cornell - Antioch CA, US
Megan Choi - El Cerrito CA, US
Halina Ewa Witkowski - Walnut Creek CA, US
Robert M. Glaeser - Berkeley CA, US

Assignee:

The Regents of the University of California - Oakland CA

International Classification:

G01N 33/559
C25B 7/00
G01N 27/447

US Classification:

204452, 204605, 204455

Abstract:

A multi-channel gel electrophoresis apparatus for efficiently collecting molecules isolated by gel electrophoresis so they can be further analyzed, identified, or used as reagents or medications. The apparatus using a novel “tagless” strategy that combines multi-dimensional separation of endogenous complexes with mass spectrometric monitoring of their composition. In this procedure, putative protein complexes are identified based on the co-migration of collections of polypeptides through multiple orthogonal separation steps. A majority of proteins are shown to remain in stable complexes during fractionation of a crude extract through three chromatographic steps.

US Patent:

20130037712, Feb 14, 2013

Filed:

Jun 4, 2012

Appl. No.:

13/487831

Inventors:

Robert GLAESER - Berkeley CA, US
Jian Jin - Berkeley CA, US
Radostin Danev - Germany, DE
Holger Mueller - Orinda CA, US
John Spence - Temple AZ, US

Assignee:

REGENTS OF THE UNIVERSITY OF CALIFORNIA - Oakland CA

International Classification:

H01J 37/22

US Classification:

250307, 250311

Abstract:

An optical phase plate system and method for enhancing phase contrast in electron beam imaging includes a transmission electron microscope (TEM) having a back focal plane; an optical cavity having a high internal surface reflectance, the center of the optical cavity located at the back focal plane of the TEM, the optical cavity having first and second ports arranged oppositely along a symmetrical axis of the optical cavity to admit an electron beam provided by the TEM through the first port to pass through and focus at the center of the optical cavity, and to exit through the second port, and wherein the optical cavity further has an optical port on an axis transverse to and intersecting the electron beam axis to admit a laser beam; a laser coupled to the optical cavity to provide a laser beam of a selected wavelength to enter the optical cavity through the optical port, wherein the laser beam is multiply reflected from the high internal surface reflectance to provide a high intensity standing wave optical phase plate focused at the back focal plane of the TEM to cause a modulation of the electron beam; and an image plane of the TEM placed opposite the second port of the optical cavity to receive the electron beam modulated by the high intensity standing wave optical phase plate.

US Patent:

20130099115, Apr 25, 2013

Filed:

Dec 7, 2012

Appl. No.:

13/708521

Inventors:

The Regents of the University of California - Oakland CA, US
Rossana Cambie - Moraga CA, US
Jian Jin - Berkeley CA, US

Assignee:

The Regents of the University of California - Oakland CA

International Classification:

H01J 37/26

US Classification:

250307, 250311

Abstract:

A variant of the Foucault (knife-edge) aperture is disclosed that is designed to provide single-sideband (SSB) contrast at low spatial frequencies but retain conventional double-sideband (DSB) contrast at high spatial frequencies in transmission electron microscopy. The aperture includes a plate with an inner open area, a support extending from the plate at an edge of the open area, a half-circle feature mounted on the support and located at the center of the aperture open area. The radius of the half-circle portion of reciprocal space that is blocked by the aperture can be varied to suit the needs of electron microscopy investigation. The aperture is fabricated from conductive material which is preferably non-oxidizing, such as gold, for example.