Dupont 2015 - 2016
Principal Investigator
Dupont 2003 - 2008
Senior Research Investigator
Dupont 1998 - 2002
Research Investigator
Boyce Thompson Institute 1993 - 1998
Nih Postdoctoral Fellow
Education:
University of Kentucky 1988 - 1993
Doctorates, Doctor of Philosophy, Molecular Biology, Philosophy
Peking University 1978 - 1986
Master of Science, Masters, Bachelors, Bachelor of Science, Biology
Nanyang Technological University
Master of Science, Masters, Engineering
Thompson Institute
Skills:
Molecular Biology Biochemistry Protein Chemistry Protein Expression Genomics Metabolic Engineering Protein Engineering Chemistry Molecular Genetics Recombinant Dna R&D Genetic Engineering Metabolomics Rna Biology Life Sciences Biotechnology Proteinomics Research and Development
E. I. du Pont de Nemours and Company - Wilmington DE
International Classification:
C12N 1587
US Classification:
800288, 800278, 530300, 530324, 530350
Abstract:
The invention provides methods for the production of silks and silk-like proteins (SLPs) in green plants. Expression of SLPs has been achieved in both seed and leaf tissue in green plants.
E. I. du Pont de Nemours and Company - Wilmington DE
International Classification:
A01H009/00
US Classification:
800295
Abstract:
The invention provides methods for the production of silks and silk-like proteins (SLP's) in green plants. Expression of SLP's has been achieved in both seed and leaf tissue in green plants.
The present invention provides methods for the conditional or regulated expression of a site-specific recombinase using split intein-mediated protein splicing. This enhances the temporal and tissue-specificity of trait gene expression and allows for fine tuning of expression specificity.
Robel Y. Kahsay - Wilmington DE, US Min Qi - Hockessin DE, US Luan Tao - Wallingford PA, US Paul V. Viitanen - West Chester PA, US Jianjun Yang - Hockessin DE, US
Assignee:
E I du Pont de Nemours and Company - Wilmington DE
Narendra Yadav - Chadds Ford PA, US Jianjun Yang - Hockessin DE, US
International Classification:
A01H001/00 C12N015/82 C12N009/00
US Classification:
800/288000, 435/183000, 435/468000
Abstract:
The present invention provides methods for intein-mediated protein splicing, particularly in plants. This permits in vivo and in vitro synthesis of homogeneous and large multi-functional hybrid protein polymers and circular proteins. Additionally, methods are provided which are suitable for the regulation of transgene expression, such that a particular transgene is expressed only under selected environmental conditions, in selected plant tissues, at selected development stages, or in selected plant generations.
The present invention provides methods for the accumulation of translocated proteins through targeting to the apoplast, ER lumen, and vacuoles of plants. The methods proceed by providing a consisting of a DNA construct comprising a sporamin signal sequence and a target protein, and optionally, a sporamin propeptide and/or an ER retention signal, according to the specific tissue of the plant in which the translocated protein is expressed and targeted. The work disclosed herein represents the first systematic determination of each respective location's capacity for translocated protein accumulation in different plant tissues.
Narendra Yadav - Chadds Ford PA, US Jianjun Yang - Hockessin DE, US
International Classification:
A01H001/00 C12N015/82 C12N005/04
US Classification:
800/288000, 435/069100, 435/320100, 435/419000
Abstract:
The present invention provides methods for intein-mediated protein splicing, particularly in plants. This permits in vivo and in vitro synthesis of homogeneous and large multi-functional hybrid protein polymers and circular proteins. Additionally, methods are provided which are suitable for the regulation of transgene expression, such that a particular transgene is expressed only under selected environmental conditions, in selected plant tissues, at selected development stages, or in selected plant generations.
Peptide-Based Diblock And Triblock Dispersants And Diblock Polymers
John O'Brien - Oxford PA, US Jianjun Yang - Hockessin DE, US
International Classification:
C03C017/00 C09D005/00 C07K002/00
US Classification:
523160000, 523161000, 530300000
Abstract:
Peptides have been identified that bind with high affinity to common ink pigments and to various print media, including paper and fabrics. These peptides were used to prepare diblock and triblock dispersants for coating applications, including ink jet printing, and diblock polymers for treating paper and fabrics. The diblock dispersants consist of a pigment-binding peptide coupled to a hydrophilic linker, a pigment-binding peptide coupled to a print medium-binding peptide or a print medium-binding peptide coupled to a hydrophobic linker. The diblock polymers consist of a print medium-binding peptide coupled to a hydrophilic linker or a benefit agent. The triblock dispersants consist of a pigment-binding peptide coupled to a hydrophilic linker, which is coupled to a print medium-binding peptide.