Jing Cheng

US Patent:

20190376060, Dec 12, 2019

Filed:

Mar 13, 2018

Appl. No.:

16/486586

Inventors:

- Cambridge MA, US
Alejandro Chavez - New York NY, US
Jing Cheng - Mountain View CA, US
Benjamin W. Pruitt - Cambridge MA, US

Assignee:

President and Fellows of Harvard College - Cambridge MA

International Classification:

C12N 15/11
C12N 9/22
C07K 14/31
C07K 14/315
C12N 15/86

Abstract:

The present disclosure provides methods and compositions of modulating expression of a target nucleic acid sequence in a cell. The method comprises introducing into the cell a nucleic acid sequence encoding a Cas9 fusion protein and a guide RNA, wherein the Cas9 fusion protein and the guide RNA are expressed and co-localize at a target site and modulate the expression of the target nucleic acid sequence.