Skills:
Skills Proficient in protein purification, ELISA, western blotting, TUNEL, immunohistochemistry, immunofluorescence staining, and flow cytometry. Proficient in cell (including primary cells) culture techniques, immortalization, transduction and transfection. Proficient in bacteria culturing, sterile technique, transformation, subcloning, gel electrophoresis, restriction digestion/ligation of DNA, mutagenesis, isolation of RNA and DNA, PCR, and real-time RT-PCR. Proficient in animal (murine) handling, breeding, genotyping, subcutaneous tumor model and surgeries. Proficient in GC, spectrophotometer, use of radioisotopes Superb understanding of statistical approaches used in experimental design, assay development and data processing. Fluent in English and Chinese. Proficient in Word, Excel, Powerpoint, Prism, and Endnote.