Jing Lu

US Patent:

7198894, Apr 3, 2007

Filed:

Nov 30, 2001

Appl. No.:

09/998481

Inventors:

Francis Barany - New York NY, US
Weiguo Cao - Central SC, US
Jianmin Huang - Jackson Heights NY, US
Jing Lu - Central SC, US

Assignee:

Cornell Research Foundation, Inc. - Ithaca NY

International Classification:

C12Q 1/68
C12P 19/34

US Classification:

435 6, 435 911, 435 912, 435 9152, 204450

Abstract:

The present invention is a method for detecting DNA sequence differences including single nucleotide mutations or polymorphisms, one or more nucleotide insertions, and one or more nucleotide deletions. Labeled heteroduplex PCR fragments containing base mismatches are prepared. Endonuclease cleaves the heteroduplex PCR fragments both at the position containing the variation (one or more mismatched bases) and to a lesser extent, at non-variant (perfectly matched) positions. Ligation of the cleavage products with a DNA ligase corrects non-variant cleavages and thus substantially reduces background. This is then followed by a detection step in which the reaction products are detected, and the position of the sequence variations are determined.

US Patent:

7807431, Oct 5, 2010

Filed:

Mar 5, 2007

Appl. No.:

11/682172

Inventors:

Francis Barany - New York NY, US
Weiguo Cao - Central SC, US
Jianmin Huang - Jackson Heights NY, US
Jing Lu - Tucker GA, US

Assignee:

Cornell Research Foundation, Inc. - Ithaca NY

International Classification:

C12N 9/22

US Classification:

435199, 536 232

Abstract:

The present invention is a method for detecting DNA sequence differences including single nucleotide mutations or polymorphisms, one or more nucleotide insertions, and one or more nucleotide deletions. Labeled heteroduplex PCR fragments containing base mismatches are prepared. Endonuclease cleaves the heteroduplex PCR fragments both at the position containing the variation (one or more mismatched bases) and to a lesser extent, at non-variant (perfectly matched) positions. Ligation of the cleavage products with a DNA ligase corrects non-variant cleavages and thus substantially reduces background. This is then followed by a detection step in which the reaction products are detected, and the position of the sequence variations are determined.

US Patent:

7960159, Jun 14, 2011

Filed:

Sep 2, 2010

Appl. No.:

12/874255

Inventors:

Francis Barany - New York NY, US
Weiguo Cao - Central SC, US
Jianmin Huang - Jackson Heights NY, US
Jing Lu - Tucker GA, US

Assignee:

Corneil Research Foundation, Inc. - Ithaca NY

International Classification:

C12N 9/22

US Classification:

435199, 536 232

Abstract:

The present invention is a method for detecting DNA sequence differences including single nucleotide mutations or polymorphisms, one or more nucleotide insertions, and one or more nucleotide deletions. Labeled heteroduplex PCR fragments containing base mismatches are prepared. Endonuclease cleaves the heteroduplex PCR fragments both at the position containing the variation (one or more mismatched bases) and to a lesser extent, at non-variant (perfectly matched) positions. Ligation of the cleavage products with a DNA ligase corrects non-variant cleavages and thus substantially reduces background. This is then followed by a detection step in which the reaction products are detected, and the position of the sequence variations are determined.