Joanne Recchia

US Patent:

48001660, Jan 24, 1989

Filed:

Jun 6, 1986

Appl. No.:

6/871513

Inventors:

Marcus J. Horn - Cambridge MA
Joanne Recchia - Norwood MA

Assignee:

Applied Protein Technologies, Inc. - Cambridge MA

International Classification:

G01N 3368

US Classification:

436 55

Abstract:

A method for monitoring a solid-phase peptide synthesis for free amino ends of unreacted peptide chains, the monitoring occurring at the end of each addition of a blocked amino acid to the peptide chains which are anchored to the solid phase. The method includes reacting the solid phase with a monitoring agent that forms a covalent bond with unblocked amino groups. The solid phase is then washed to remove the unreacted monitoring agent. A cleaving reagent is then used to selectively remove the covalently bound monitoring reagent from the ends of the unblocked peptide chains, while leaving the blocked chains intact. The amount of monitoring agent thus removed is then quantitatively measured to determine what proportion of the initial peptide chains failed to react with the blocked amino acid. Preferred monitoring agents for use in this process include trityl (triphenylmethyl) -based groups, and particularly preferred agents are trityl, monomethoxytrityl, dimethoxytrityl, and trimethoxytrityl chlorides.

US Patent:

56503989, Jul 22, 1997

Filed:

Jun 2, 1995

Appl. No.:

8/459502

Inventors:

Charlotte A. Kensil - Milford MA
Sean Soltysik - Worcester MA
Dante J. Marciani - Birmingham AL
Joanne Recchia - Blackstone MA

Assignee:

Cambridge Biotech Corporation - Worcester MA

International Classification:

A61K 3170
A61K 31705
A61K 3160
A61K 3156

US Classification:

514 25

Abstract:

Disclosed herein are modified saponins or fractions thereof obtainable by modification of a crude Quillaja saponaria extract or by modification of purified saponins obtainable from a crude Quillaja saponaria extract useful in pharmaceutical compositions for enhancing the transport of pharmacologically active substances across mucous membranes of an animal. Also disclosed are modified saponins having reduced irritability wherein the aldehyde group is reduced and the fatty acid moiety may be removed by hydrolysis. Also disclosed are pharmaceutical compositions comprising the modified saponins of the invention.