Joe William Gray

US Patent:

6066453, May 23, 2000

Filed:

Aug 8, 1997

Appl. No.:

8/908855

Inventors:

Daniel Pinkel - Walnut Creek CA
Donna G. Albertson - Lafayette CA
Joe W. Gray - San Francisco CA

Assignee:

The Regents of the University of California - Oakland CA

International Classification:

C12Q 168
G01N 33574
G01N 3353
A01N 5508

US Classification:

435 6

Abstract:

The present invention relates to DNA sequences from regions of copy number change on chromosome 20. The sequences can be used in hybridization methods for the identification of chromosomal abnormalities associated with various diseases.

US Patent:

6335167, Jan 1, 2002

Filed:

May 14, 1999

Appl. No.:

09/311835

Inventors:

Daniel Pinkel - Walnut Creek CA
Joe W. Gray - San Francisco CA
Anne Kallioniemi - Rockville MD
Frederic Waldman - San Francisco CA
Masaru Sakamoto - Tokyo, JP

Assignee:

The Regents of the University of California - Oakland CA

International Classification:

C12Q 168

US Classification:

435 6, 435 712, 536 231, 536 243

Abstract:

Disclosed are new methods comprising the use of in situ hybridization to detect abnormal nucleic acid sequence copy numbers in one or more genomes wherein repetitive sequences that bind to multiple loci in a reference chromosome spread are either substantially removed and/or their hybridization signals suppressed. The invention termed Comparative Genomic Hybridization (CGH) provides for methods of determining the relative number of copies of nucleic acid sequences in one or more subject genomes or portions thereof (for example, a tumor cell) as a function of the location of those sequences in a reference genome (for example, a normal human genome). The intensity(ies) of the signals from each labeled subject nucleic acid and/or the differences in the ratios between different signals from the labeled subject nucleic acid sequences are compared to determine the relative copy numbers of the nucleic acid sequences in the one or more subject genomes as a function of position along the reference chromosome spread. Amplifications, duplications and/or deletions in the subject genome(s) can be detected. Also provided is a method of determining the absolute copy numbers of substantially all RNA or DNA sequences in subject cell(s) or cell population(s).

US Patent:

6417506, Jul 9, 2002

Filed:

Aug 17, 2000

Appl. No.:

09/642243

Inventors:

Daniel Pinkel - Walnut Creek CA
Joe Gray - San Francisco CA
Donna G. Albertson - Lafayette CA

Assignee:

The Regents of the University of California - Oakland CA
Medical Research Council - London

International Classification:

C25D 1300

US Classification:

250216, 25022711, 204603, 600473

Abstract:

The invention relates to the fabrication and use of biosensors comprising a plurality of optical fibers each fiber having attached to its âsensor endâ biological âbinding partnersâ (molecules that specifically bind other molecules to form a binding complex such as antibody-antigen, lectin-carbohydrate, nucleic acid-nucleic acid, biotin-avidin, etc. ). The biosensor preferably bears two or more different species of biological binding partner. The sensor is fabricated by providing a plurality of groups of optical fibers. Each group is treated as a batch to attach a different species of biological binding partner to the sensor ends of the fibers comprising that bundle. Each fiber, or group of fibers within a bundle, may be uniquely identified so that the fibers, or group of fibers, when later combined in an array of different fibers, can be discretely addressed. Fibers or groups of fibers are then selected and discretely separated from different bundles. The discretely separated fibers are then combined at their sensor ends to produce a high density sensor array of fibers capable of assaying simultaneously the binding of components of a test sample to the various binding partners on the different fibers of the sensor array.

US Patent:

6465182, Oct 15, 2002

Filed:

Apr 29, 1999

Appl. No.:

09/302056

Inventors:

Joe Gray - San Francisco CA
Dan Pinkel - Walnut Creek CA
Donna Albertson - Lafayette CA
Colin Collins - San Rafael CA
Russell Baldocchi - San Francisco CA

Assignee:

The Regents of the University of California - Oakland CA

International Classification:

C12Q 168

US Classification:

435 6, 435 911, 435 912, 536 243, 536 2433, 536 266, 536 231

Abstract:

The present invention provides methods of determining relative copy number of target nucleic acid sequences and precise mapping of chromosomal abnormalities associated with disease. The methods of the invention use target nucleic acid sequences immobilized on a solid surface, to which a sample comprising two sets of differentially labeled nucleic acid sequences are hybridized. The hybridization of the labeled nucleic acid sequences to the solid surface is then detected using standard techniques.

US Patent:

6475732, Nov 5, 2002

Filed:

Jul 17, 2000

Appl. No.:

09/617322

Inventors:

Laleh Shayesteh - Foster City CA
Joe W. Gray - San Francisco CA

Assignee:

The Regents of the University of California - Oakland CA

International Classification:

C12Q 168

US Classification:

435 6

Abstract:

The present invention provides new probes for the detection of chromosomal alterations associated with cancer, particularly ovarian cancer. The probes bind selectively with target nucleic acid sequences at 3q26.

US Patent:

6537761, Mar 25, 2003

Filed:

Jul 13, 2000

Appl. No.:

09/615440

Inventors:

Laleh Shayesteh - Foster City CA
Joe W. Gray - San Francisco CA

Assignee:

The Regents of the University of California - Oakland CA

International Classification:

G01N 3353

US Classification:

435 71, 435 72, 530350, 5303871

Abstract:

The present invention provides new probes for the detection of chromosomal alterations associated with cancer, particularly ovarian cancer. The probes bind selectively with target nucleic acid sequences at 3 26.

US Patent:

6562565, May 13, 2003

Filed:

Jun 26, 1996

Appl. No.:

08/670953

Inventors:

Daniel Pinkel - Walnut Creek CA
Donna Albertson - Cambridge, GB
Joe W. Gray - San Francisco CA

Assignee:

The Regents of the University of California - Oakland CA
Medical Research Council - London

International Classification:

C12Q 168

US Classification:

435 6, 435 5, 435 911, 435 912, 536 243

Abstract:

The present invention provides methods of determining relative copy number of target nucleic acids and precise mapping of chromosomal abnormalities associated with disease. The methods of the invention use target nucleic acids immobilized on a solid surface, to which a sample comprising two sets of differentially labeled nucleic acids are hybridized. The hybridization of the labeled nucleic acids to the solid surface is then detected using standard techniques.

US Patent:

6610499, Aug 26, 2003

Filed:

Aug 31, 2000

Appl. No.:

09/652873

Inventors:

Mack J. Fulwyler - Sarasota FL
Joe W. Gray - San Francisco CA

Assignee:

The Regents of the University of California - Oakland CA

International Classification:

G01N 3353

US Classification:

435 71, 436 52, 436518, 436534, 4352865, 422 81, 422 82

Abstract:

The invention provides methods and devices for detecting the presence of one or more target analytes in a sample employing a channel having affixed therein one or more binding partners for each target analyte. Assays are carried out by transporting the sample through the channel to each successive binding partner so that target analyte present in said sample binds to the corresponding binding partner. The sample is then transported beyond the binding partner(s), followed by detection of any target analyte bound to each binding partner. In one embodiment, binding efficiency is increased by the use of segmented transport, wherein a first bolus or bubble of a fluid that is immiscible with the sample precedes the sample during transport and a second bolus or bubble of a fluid that is immiscible with the sample follows the sample. Many configurations are possible for the device of the invention. A preferred device includes: a substrate with a channel formed in its surface, and a cover element that overlies and seals the channel.