John H Albers

US Patent:

57121575, Jan 27, 1998

Filed:

Dec 20, 1994

Appl. No.:

8/360234

Inventors:

Santica M. Marcovina - Seattle WA
John J. Albers - Seattle WA

Assignee:

University of Washington - Seattle WA

International Classification:

C07K 1618
C12N 520

US Classification:

435337

Abstract:

The present invention relates to monoclonal antibodies and a method for the quantification of apolipoprotein(a) and lipoprotein(a) present in human body fluids. The method is not sensitive to the presence of plasminogen or the apo(a) kringle 4 repeats. The assay involves the addition of the test sample to an immobilized anti-apo(a) antibody and forming a first immobilized complex of apolipoprotein(a)/anti-apo(a) first antibody, contacting first immune complex with an anti-apo(a) monoclonal antibody specific for apo(a) with no crossreactivity to plasminogen and which does not identify the kringle 4 type 2 apo(a) repeats and thereafter quantitating apo(a) based on the amount of bound anti-apo(a) monoclonal antibody. Immobilized goat or rabbit anti-human apo(a) antibody can be employed as the first capture antibody and the mouse anti-apo(a) monoclonal antibody as second antibody. The amount of anti-apo(a) monoclonal antibody bound is quantitated through binding of a third antibody enzyme conjugant, e. g.

US Patent:

56100191, Mar 11, 1997

Filed:

Jun 5, 1995

Appl. No.:

8/462733

Inventors:

Joseph R. Day - Brier WA
John J. Albers - Seattle WA
Janet L. Adolphson - Mountlake Terrace WA

Assignee:

ZymoGenetics, Inc. - Seattle WA

International Classification:

C12N 910
C12N 912
C12N 1100
C12Q 148

US Classification:

435 71

Abstract:

Isolated polynucleotide molecules encoding mammalian phospholipid transfer proteins (PLTP) and phospholipid transfer protein polypeptides are disclosed. The DNA molecules are transformed or transfected into host cells and the cells cultured to produce recombinant PLTP and PLTP polypeptides. PLTP and PLTP polypeptides may be combined with a pharmaceutically acceptable vehicle and administered to patients to regulate phospholipid transfer activity and thereby obtain a more favorable lipoprotein profile in the blood. The proteins and polypeptides may also be used within methods to measure phospholipid transfer activity or identify inhibitors of phospholipid transfer activity.

US Patent:

56228430, Apr 22, 1997

Filed:

Dec 30, 1993

Appl. No.:

8/176402

Inventors:

Joseph R. Day - Brier WA
John J. Albers - Seattle WA
Janet L. Adolphson - Mountlake Terrace WA

Assignee:

Zymogenetics, Inc. - Seattle WA
University of Washington - Seattle WA

International Classification:

C07K 14435
C12N 1512

US Classification:

435 696

Abstract:

Isolated polynucleotide molecules encoding mammalian phospholipid transfer proteins (PLTP) and phospholipid transfer protein polypeptides are disclosed. The DNA molecules are transformed or transfected into host cells and the cells cultured to produce recombinant PLTP and PLTP polypeptides. PLTP and PLTP polypeptides may be combined with a pharmaceutically acceptable vehicle and administered to patients to regulate phospholipid transfer activity and thereby obtain a more favorable lipoprotein profile in the blood. The proteins and polypeptides may also be used within methods to measure phospholipid transfer activity or identify inhibitors of phospholipid transfer activity.