John E Dueber

US Patent:

20050004347, Jan 6, 2005

Filed:

Jul 3, 2003

Appl. No.:

10/613380

Inventors:

Wendell Lim - San Francisco CA, US
John Dueber - San Francisco CA, US
Brian Yeh - San Francisco CA, US

International Classification:

G06F019/00
G01N033/48
G01N033/50
C12N009/00

US Classification:

530350000, 702019000, 435183000

Abstract:

Protein logic gates are made from autoregulated fusion proteins comprising an output domain and a plurality of input domains, wherein at least one of the input domains is heterologous to the output domain, and the input domains interact with each other to allosterically and external, ligand-dependently regulate the output domain. The output domain may be constitutively active, and in the absence of the ligand, the input domains interact to inhibit the output domain. The activity of the output domain is user discretionary, and may include activities that are catalytic, label-generative, metabolic-regulative, apototic, specific-binding, etc. Multiple input domains can cooperatively regulate the fusion protein in a wide variety of functionalities, including as an OR-gate, an AND-gate, and an AND-NOT-gate. The gates may be incorporated into cells and therein used to modulate cell function.

US Patent:

20110008829, Jan 13, 2011

Filed:

Feb 26, 2009

Appl. No.:

12/918725

Inventors:

John E. Dueber - Oakland CA, US
Jay D. Keasling - Berkeley CA, US
Gabriel C. Wu - Daly City CA, US
Ghulam Reza Kahn Malmirchegini - Livermore CA, US

International Classification:

C12P 21/02
C07H 21/00
C12N 15/63
C12N 1/21

US Classification:

435 681, 536 231, 536 232, 4353201, 43525233

Abstract:

The present invention provides methods of producing a product or product precursor of a biosynthetic pathway in a genetically modified host cell. The present invention also provides genetically modified host cells comprising nucleic acids encoding a scaffold polypeptide and nucleic acids comprising nucleotide sequences encoding two or more enzymes in a biosynthetic pathway. The present invention further provides nucleic acids comprising nucleotide sequences encoding scaffold polypeptides, for use in a subject method.

US Patent:

20120135524, May 31, 2012

Filed:

Apr 15, 2011

Appl. No.:

13/088288

Inventors:

Joshua M. Gilmore - Emeryville CA, US
J. Christopher Anderson - San Francisco CA, US
John E. Dueber - San Francisco CA, US

Assignee:

THE REGENTS OF THE UNIVERSITY OF CALIFORNIA - Oakland CA

International Classification:

C12N 15/01
C12N 15/70
C12N 1/21
C12N 15/11

US Classification:

435441, 536 231, 4353201, 43525233

Abstract:

The present invention provides for a recombinant nucleic acid comprising a nucleotide sequence comprising a plurality of constructs, wherein each construct independently comprises a nucleotide sequence of interest flanked by a pair of recombinase recognition sequences. Each pair of recombinase recognition sequences is recognized by a distinct recombinase. Optionally, each construct can, independently, further comprise one or more genes encoding a recombinase capable of recognizing the pair of recombinase recognition sequences of the construct. The recombinase can be an orthogonal (non-cross reacting), site-selective recombinase (OSSR).

US Patent:

20170306301, Oct 26, 2017

Filed:

Sep 24, 2015

Appl. No.:

15/507711

Inventors:

- Oakland CA, US
- Montreal, CA
John E. Dueber - San Francisco CA, US
William C. DeLoache - Oakland CA, US
Zachary N. Russ - Berkeley CA, US
P. James Scrivens - St. Constant, CA

Assignee:

The Regents of the University of California - Oakland CA

International Classification:

C12N 9/02
C12P 17/10
C12P 13/22
C12P 13/00
C12Q 1/26
C12P 7/24

Abstract:

The present disclosure provides a variant tyrosine hydroxylase that provides for increased production of L-DOPA in a host cell that expresses the tyrosine hydroxylase. The present disclosure provides nucleic acids encoding the variant tyrosine hydroxylase, and host cells genetically modified with the nucleic acids. The present disclosure provides methods of making L-DOPA in a host cell. The present disclosure provides methods of making a benzylisoquinoline alkaloid (BIA), or a BIA precursor. The present disclosure provides methods of detecting L-DOPA level in a cell. The present disclosure provides methods of identifying tyrosine hydroxylase variants that provide for increased L-DOPA production; and methods of identifying gene products that provide for increased tyrosine production.