John C Flanagan

US Patent:

20090081688, Mar 26, 2009

Filed:

Sep 17, 2008

Appl. No.:

12/284163

Inventors:

Yuling Luo - San Ramon CA, US
Shiping Chen - Fremont CA, US
Huei-Yu Fay Wang - San Francisco CA, US
John James Flanagan - Walnut Creek CA, US

Assignee:

Advanced Cell Diagnostics - Fremont CA

International Classification:

C12Q 1/68

US Classification:

435 6

Abstract:

Methods of detecting multiple nucleic acid targets in single cells through indirect capture of labels to the nucleic acids are provided. Methods of assaying the relative levels of nucleic acid targets through normalization to levels of reference nucleic acids are also provided. Methods of detecting individual cells, particularly rare cells from large heterogeneous cell populations, through detection of nucleic acids are described. Related compositions, systems, and kits are also provided.

US Patent:

20130023433, Jan 24, 2013

Filed:

Sep 28, 2010

Appl. No.:

13/498249

Inventors:

Yuling Luo - San Ramon CA, US
John James Flanagan - Walnut Creek CA, US
Nan Su - San Ramon CA, US
Huei-Yu Fay Wang - San Francisco CA, US

International Classification:

C12Q 1/68
C40B 30/04
G01N 33/53

US Classification:

506 9, 436501, 435 611

Abstract:

The invention relates to methods of detecting nucleic acids, including methods of detecting one or more target nucleic acid sequences in multiplex branched-chain DNA assays, are provided. Nucleic acids captured on a solid support or suspending cells are detected, for example, through cooperative hybridization events that result in specific association of a label with the nucleic acids. The invention further relates to methods to improve probe hybridization specificity and their application in genotyping. The invention also relates to in situ detection of mis-joined nucleic acid sequences. The invention relates to reducing false positive signals and improve signal-to-background ratio in hybridization-based nucleic acid detection assay. The invention further relates to method to improve specificity in hybridization based nucleic acid using co-location probes. Compositions, tissue slides, sample of suspended cells, kits, and systems related to the methods are also described.

US Patent:

20130171621, Jul 4, 2013

Filed:

Jan 31, 2011

Appl. No.:

13/575936

Inventors:

Yuling Luo - San Ramon CA, US
Huei-Yu Wang - San Francisco CA, US
John James Flanagan - Walnut Creek CA, US
Shiping Chen - Fremont CA, US

International Classification:

C12Q 1/68

US Classification:

435 5, 435 611

Abstract:

Methods of detecting the presence or absence of a class of nucleic acid targets in single cells through direct or indirect capture of labels to the nucleic acids are provided, where such labels to the class of nucleic acid targets are indistinguishable from each other. Also described are methods of detecting individual cells, particularly a cell of a specific type from large heterogeneous cell populations, through detection of one or more of nucleic acid targets, where the labels to the one or more of nucleic acid targets are indistinguishable from each other. Related kits are also described.

US Patent:

20130196350, Aug 1, 2013

Filed:

Jan 31, 2013

Appl. No.:

13/755234

Inventors:

Michelle Delanoy - Danville CA, US
Audrey Arjomandi - San Francisco CA, US
John Flanagan - Walnut Creek CA, US

Assignee:

Bio-Rad Laboratories, Inc. - Hercules CA

International Classification:

G01N 33/68
G01N 33/74

US Classification:

435 723

Abstract:

Improved ovarian cancer assays are provided.

US Patent:

6326028, Dec 4, 2001

Filed:

Oct 30, 1998

Appl. No.:

9/183238

Inventors:

Thierry Nivaggioli - San Francisco CA
George Colegrove - San Diego CA
John Flanagan - Neshanic Station NJ

Assignee:

Monsanto Company - St. Louis MO

International Classification:

A61K 928
A61K 934
A61K 936

US Classification:

424481

Abstract:

This invention relates to coatings employed to coat medicinal tablets. Such tablets include but are not limited to small pellet(s) of medication to be taken orally. In particular this invention relates to the use of alginates and/or gellan gum, mixtures thereof and the like as tablet coatings. More in particular, this invention relates to the use of alginate and gellan gum as tablet coatings for tablets which are useful for humans including coating(s) on medicinal tablets and to a process for making such coatings.

US Patent:

20210275484, Sep 9, 2021

Filed:

Mar 9, 2018

Appl. No.:

16/492494

Inventors:

- Toronto, CA
- Oakland CA, US
John G. Flanagan - Berkeley CA, US
Karsten Gronert - Walnut Creek CA, US
Jessica Wei - Berkeley CA, US

International Classification:

A61K 31/202
A61K 31/557
A61P 25/28

Abstract:

Methods and compositions for inhibiting or preventing neurodegeneration, specifically hippocampal, cortical, and/or retinal ganglion cell neurons (RGC), and degeneration and/or cell loss or treating related disorders and diseases comprising administering to a subject an effective amount of one or more lipoxin compounds and/or lipoxin analogues such that degeneration and/or cell loss of neurons is inhibited or prevented.

US Patent:

20210032689, Feb 4, 2021

Filed:

Aug 18, 2020

Appl. No.:

16/996180

Inventors:

- Newark CA, US
Shiping Chen - Fremont CA, US
Huei-Yu Fay Wang - San Francisco CA, US
John James Flanagan - Walnut Creek CA, US

International Classification:

C12Q 1/6841

Abstract:

Methods of detecting multiple nucleic acid targets in single cells through indirect capture of labels to the nucleic acids are provided. Methods of assaying the relative levels of nucleic acid targets through normalization to levels of reference nucleic acids are also provided. Methods of detecting individual cells, particularly rare cells from large heterogeneous cell populations, through detection of nucleic acids are described. Related compositions, systems, and kits are also provided.

US Patent:

20200399689, Dec 24, 2020

Filed:

Jun 4, 2020

Appl. No.:

16/892826

Inventors:

- Newark CA, US
John James FLANAGAN - Walnut Creek CA, US
Nan SU - San Ramon CA, US
Huei-Yu Fay WANG - San Francisco CA, US

Assignee:

ADVANCED CELL DIAGNOSTICS, INC. - Newark CA

International Classification:

C12Q 1/6841

Abstract:

The invention relates to methods of detecting nucleic acids, including methods of detecting one or more target nucleic acid sequences in multiplex branched-chain DNA assays, are provided. Nucleic acids captured on a solid support or suspending cells are detected, for example, through cooperative hybridization events that result in specific association of a label with the nucleic acids. The invention further relates to methods to improve probe hybridization specificity and their application in genotyping. The invention also relates to in situ detection of mis-joined nucleic acid sequences. The invention relates to reducing false positive signals and improve signal-to-background ratio in hybridization-based nucleic acid detection assay. The invention further relates to method to improve specificity in hybridization based nucleic acid using co-location probes. Compositions, tissue slides, sample of suspended cells, kits, and systems related to the methods are also described.