John W Hamman

US Patent:

56771622, Oct 14, 1997

Filed:

May 1, 1995

Appl. No.:

8/432955

Inventors:

Jinsheng Zou - Bronx NY
John Hamman - Baltimore MD
Gerard Marx - New York NY
Bernard Horowitz - New Rochelle NY

Assignee:

New York Blood Center, Inc. - New York NY

International Classification:

C12N 974
C12N 704
C12N 706
A61K 3848

US Classification:

435214

Abstract:

The present invention provides a method for activating prothrombin to thrombin in a prothrombin complex composition comprising at least prothrombin, Factors V, VII, IX, and X, and phospholipid, comprising the steps of: (a) cold-incubating the prothrombin complex composition with 2-15 mM of Ca. sup. 2+ ions at a pH of 6. 5-8. 0 and at a temperature of 2. degree. -8. degree. C. until Factor VII contained in the composition is activated; and (b) incubating the cold-incubated composition at a pH of 6. 5-8. 0 and at a temperature of 10. degree. -25. degree. C. for a period of time sufficient to activate prothrombin contained in the composition to thrombin.