John Raymond Hammond

US Patent:

8637653, Jan 28, 2014

Filed:

Dec 22, 2009

Appl. No.:

12/645027

Inventors:

John Hammond - Laurel MD, US
Hyoun Sub Lim - Ellicott City MD, US
Leslie L. Domier - Urbana IL, US

Assignee:

The United States of America, as represented by the Secretary of Agriculture - Washington DC

International Classification:

C12N 7/01
C12N 15/33
C12N 15/82
C12N 15/87
C12N 5/10
C12N 15/113
A01H 5/00

US Classification:

536 2372, 4353201, 435419, 536 245, 800280, 800285, 800288

Abstract:

We have developed a versatile plant viral vector system based on Alternanthera mosaic virus (AltMV), suitable for infection by agroinfiltration or in vivo T7 transcripts from the same clone; agroinfection is enhanced by coinfiltration of a T7 RNA polymerase construct. Variants adapted for efficient protein expression, or for virus-induced gene silencing (VIGS), are based on a specific amino acid substitution (L88P) in the triple gene block 1 (TGB1) protein affecting RNA silencing suppression. A bipartite delivery system developed for AltMV delivers replicase (RdRp) functions separately from movement and encapsidation (TGB and coat protein, CP) functions by agroinfiltration, resulting in precise recombination of RdRp and TGB-CP constructs in planta. The bipartite delivery system has potential for high throughput protein expression or VIGS with the appropriate TGB1 variant, for hosts including and. Equivalent TGB1 substitutions in other potexviruses also reduced RNA silencing suppression, demonstrated with Potato virus X.

US Patent:

50432630, Aug 27, 1991

Filed:

May 24, 1990

Appl. No.:

7/527638

Inventors:

John Hammond - Laurel MD
Ramon L. Jordan - Jessup MD

International Classification:

C12Q 170
G01N 3353
G01N 33536
G01N 33569

US Classification:

435 5

Abstract:

The present invention is directed to monoclonal antibodies, and hybridomas which produce them, which are reactive with a highly conserved epitope present on potyviruses and insignificantly reactive with other plant viruses, as well methods of using these monoclonal antibodies to detect potyviruses.