John S Lin

US Patent:

20130066402, Mar 14, 2013

Filed:

Aug 17, 2012

Appl. No.:

13/588957

Inventors:

John Yu-luen Lin - Del Mar CA, US
Roger Y. Tsien - La Jolla CA, US

Assignee:

The Regents of the University of California - Oakland CA

International Classification:

C07K 19/00
A61N 5/06
C12N 15/864
C12N 15/62
C12N 15/85

US Classification:

607 88, 536 234, 435455, 4353201, 530350

Abstract:

The invention provides engineered red-shifted channelrhodopsin variants. In some embodiments, the channelrhodopsin variants are characterized by improved membrane trafficking, expression, and/or unique spectral and kinetic properties.

US Patent:

20180201655, Jul 19, 2018

Filed:

Nov 22, 2017

Appl. No.:

15/821574

Inventors:

- Oakland CA, US
John Lin - Del Mar CA, US
Roger Tsien - Eugene OR, US
Richard Ting - New York NY, US
Geraldine Tran - La Jolla CA, US

International Classification:

C07K 14/195

Abstract:

The invention provides evolved red-shifted smURFPs. In some embodiments, the smURFPs are characterized by a) increased Fluorescence of smURFP as compared to infrared FPs IFP1.4 and iRFP713; b) expressing efficiently with minimal toxicity; c) does not require a lyase to covalently attach its chromophore, wherein the chromophore is biliverdin; d) exhibit a wavelengths longer than attainable with jellyfish- or coral-derived FPs using smURFP and IFP2.0; e) allows for functional fusion to hCdt1(30/120) as compared to jellyfish- or coral-derived FPs mAG, eGFP, and mRFP1 which are nonfunctional; and f) allows for deep tissue imaging.