John R Majer

US Patent:

8377685, Feb 19, 2013

Filed:

Nov 7, 2008

Appl. No.:

12/267524

Inventors:

Ivar Meyvantsson - Madison WI, US
John Majer - Madison WI, US
Steven Hayes - Madison WI, US

Assignee:

BellBrook Labs, LLC - Madison WI

International Classification:

G01N 33/53
C12Q 1/24
C12Q 1/20
C12M 1/00
C12M 3/00
C12M 1/34

US Classification:

4352885, 435 71, 435 30, 435 33, 4352831, 422100

Abstract:

A microfluidic method and device for testing and analyzing chemotaxis by providing a stable, static fluid gradient. The device includes a sink reservoir for receiving biological cellular material and a source reservoir for receiving a chemoattractant. The biological cellular material migrates through a low fluid volume microfluidic gradient channel located between the source and sink reservoirs. The fluid in the gradient channel is static and stable due to a high fluid volume closed circuit bypass microfluidic channel also in fluid communication with the source and sink reservoirs, whereby the bypass channel relieves any pressure differential imparted across the gradient channel.

US Patent:

20040219510, Nov 4, 2004

Filed:

Jan 30, 2004

Appl. No.:

10/769578

Inventors:

Robert Lowery - Madison WI, US
John Majer - Madison WI, US

International Classification:

C12Q001/00
C12Q001/66
C12Q001/48

US Classification:

435/004000, 435/008000, 435/007100, 435/015000

Abstract:

The present invention relates to methods for detecting, quantifying and high throughput screening of donor-products and the catalytic activities generating the donor-products in group-transfer reactions. The invention further provides immunoassays, antibodies and kits that may be used to practice the methods of the invention.

US Patent:

20040259177, Dec 23, 2004

Filed:

May 6, 2004

Appl. No.:

10/839916

Inventors:

Robert Lowery - Madison WI, US
John Majer - Madison WI, US
David Beebe - Madison WI, US

International Classification:

G01N033/574
C12M001/34

US Classification:

435/007230, 435/287200

Abstract:

The present invention provides a novel microscale fluid handling system for initiating, culturing, manipulating and assaying three-dimensional multicellular assembly. The system including a microfluidic device and three-dimensional multicellular tissue surrogate assembly. The device of the invention includes at least one microfluidic channel; and at least one chamber, wherein the walls of the chamber are lined with a cell layer; and wherein fluid medium flows through each of the channels and chambers. Also, disclosed are methods for using the device to introducing test agents to the multicellular assemblies to observe biological responses thereof.

US Patent:

20080234137, Sep 25, 2008

Filed:

Dec 18, 2007

Appl. No.:

11/958965

Inventors:

Robert G. Lowery - Madison WI, US
John Majer - Madison WI, US

International Classification:

C40B 30/04
C12Q 1/00
C12Q 1/68
C07K 16/18
G01N 33/53
C12Q 1/48

US Classification:

506 9, 435 4, 435 6, 435 71, 435 15, 435 792, 5303871

Abstract:

The present invention relates to methods for detecting, quantifying and high throughput screening of donor-products and the catalytic activities generating the donor-products in group-transfer reactions. The invention further provides immunoassays, antibodies and kits that may be used to practice the methods of the invention.