John K Mayo

US Patent:

20180030125, Feb 1, 2018

Filed:

Aug 1, 2017

Appl. No.:

15/665955

Inventors:

John Mayo - Nashville TN, US
Kevin Jones - Knoxville TN, US

International Classification:

C07K 16/18
C07K 14/47
G01N 33/18
C07K 16/44
G01N 33/02
G01N 33/68
C07K 16/08

Abstract:

A device and method for detecting the presence of a target analyte in a fluid sample are provided. The device can include an inclined capillary tube for transporting a sample fluid containing a sample and functionalized magnetic beads. The functionalized magnetic beads capture the target analyte from the sample fluid. A well is coupled with the inclined capillary tube for containing a developer solution and for receiving the sample fluid. In at least one embodiment a magnet is movably attached to the inclined capillary tube for attracting the magnetic beads of the sample fluid and moving the magnetic beads into the well. A calorimeter is disposed adjacent to the well for receiving heat output from a reaction caused by an enzyme reaction associated with the captured target analyte in the developer solution thereby allowing detection and quantification of the target analyte.

US Patent:

20150160205, Jun 11, 2015

Filed:

Feb 19, 2015

Appl. No.:

14/626885

Inventors:

Franz Baudenbacher - Franklin TN, US
Raymond Mernaugh - Franklin TN, US
John Mayo - Nashville TN, US
Brad Lubbers - Madison TN, US
Robert G. Wiley - Franklin TN, US

International Classification:

G01N 33/543

Abstract:

One aspect of the present disclosure relates to a calorimeter for detecting the presence of a target analyte in a fluid sample. The calorimeter can include a support structure, a hermetically-sealed, thermally decoupled central reaction zone associated with the support structure, at least one droplet transport region, and detection electronics. The at least one droplet transport region can be associated with the support structure and configured to merge a reagent droplet with a sample droplet including the fluid sample to form a reaction droplet in the central reaction zone. The detection electronics can be in electrical and/or thermal communication with the central reaction zone and associated with the support structure. The calorimeter can be configured to detect a heat of reaction produced by a reaction event between the target analyte and a capture reagent upon formation of the reaction droplet.

US Patent:

20150079583, Mar 19, 2015

Filed:

Aug 22, 2014

Appl. No.:

14/466305

Inventors:

- Nashville TN, US
Raymond Mernaugh - Franklin TN, US
John Mayo - Nashville TN, US
Brad Lubbers - Madison TN, US

International Classification:

G01N 33/543
G01N 33/574
B01L 3/00
G01N 25/48

US Classification:

435 5, 422 51, 436501, 435 792, 4352872, 435 28, 435 27, 4352879, 4352871, 435 71, 435 72, 435 795, 435 793, 435 794, 435 612

Abstract:

One aspect of the present disclosure relates to a calorimeter for detecting the presence of a target analyte in a fluid sample. The calorimeter can include a substrate, a hermetically-sealed, thermally decoupled central reaction zone associated with the substrate, at least one droplet transport region, and detection electronics. The at least one droplet transport region can be associated with the substrate and configured to merge a reagent droplet with a sample droplet including the fluid sample to form a reaction droplet in the central reaction zone. The detection electronics can be in electrical and/or thermal communication with the central reaction zone and associated with the substrate. The calorimeter can be configured to detect a heat of reaction produced by a reaction event between the target analyte and a capture reagent upon formation of the reaction droplet.