John F Munger

US Patent:

7517647, Apr 14, 2009

Filed:

Sep 29, 2004

Appl. No.:

10/953658

Inventors:

Daniel B. Rifkin - New York NY, US
John S. Munger - New York NY, US
Justin P. Annes - New York NY, US

Assignee:

New York University - New York NY

International Classification:

C12Q 1/68
C12Q 1/66
C12P 21/02
C12N 5/10

US Classification:

435 6, 435 8, 435 691, 435373

Abstract:

A method of screening for a protein involved in the extracellular regulation of latent TGFβ activation by transducing a cell line with a retroviral cDNA library to create a reporter cell line that produces green fluorescent protein (GFP) in response to TGF-β signaling; growing individual clones created by the reporter cell line; co-culturing each individual clone with a second TGF-β reporter cell line that produces luciferase in response to TGF-β, wherein the luciferase production identifies positive clones; and identifying a mechanism of latent TGF-β activation that is employed by the positive clones. A TGFβ reporter cell line including a cell line; and a retroviral cDNA library, wherein the reporter cell line produces GFP in response to TGF-β signaling. A method of screening for gene products involved in a biological process.